Mécanisme de dommages du propylthiouracile sur la fonction spermatogénique et la qualité du sperme chez le rat via le stress du réticulum endoplasmique et intervention de Shugan Bushen Yulin Tang

  • role: First author第一作者
  • Affiliation:

    School of Basic Medical Sciences, Hubei University of Chinese Medicine, Wuhan 430065, China

    Hubei Shizhen Laboratory, Wuhan 430065, China

  • Email:1653600142@qq.com
  • Introduction:孟宇豪,在读硕士,从事方剂配伍与临床应用及中西医结合防治不孕不育研究,E-mail:1653600142@qq.com
MENG Yuhao12,  
  • Affiliation:

    Hubei Shizhen Laboratory, Wuhan 430065, China

    Laboratory Animal Center, Hubei University of Chinese Medicine, Wuhan 430065, China

JIANG Xiaocui23,  
  • role: Corresponding author通信作者
  • Affiliation:

    Hubei Shizhen Laboratory, Wuhan 430065, China

    Laboratory Animal Center, Hubei University of Chinese Medicine, Wuhan 430065, China

  • Email:531637551@qq.com
  • Introduction:萧闵,博士,副研究员,从事动物模型及中西医结合防治不孕不育研究,E-mail:531637551@qq.com
XIAO Min23*,  
  • role: Corresponding author通信作者
  • Affiliation:

    School of Basic Medical Sciences, Hubei University of Chinese Medicine, Wuhan 430065, China

    Hubei Shizhen Laboratory, Wuhan 430065, China

  • Email:43382275@qq.com
  • Introduction:王朝阳,博士,教授,从事中医藏象理论及其临床应用研究,E-mail:43382275@qq.com; *
WANG Chaoyang12*

résumé

L'objectif est d'examiner le mécanisme par lequel la formule Shugan Bushen Yulin Tang améliore la dysfonction spermatogénétique et la qualité du sperme induites par le propylthiouracile (PTU) chez le rat, sur la base de la voie de stress du réticulum endoplasmique (ERS). Méthodes : soixante rats mâles ont été répartis aléatoirement en groupe contrôle, groupe modèle, groupes basse, moyenne et haute dose de Shugan Bushen Yulin Tang, et groupe carnitine, avec 10 rats par groupe. À l'exception du groupe contrôle, les autres groupes ont reçu du PTU par gavage oral pendant 12 jours. Ensuite, les groupes traités ont reçu leurs respective administrations pendant 28 jours. Les indices organiques, les changements histopathologiques, les niveaux hormonaux, les marqueurs d'oxydation, l'apoptose cellulaire et l'expression protéique liée au ERS ont été évalués. Les résultats ont montré une détérioration dans le groupe modèle qui a été atténuée par les traitements. Conclusion : Shugan Bushen Yulin Tang améliore la dysfonction spermatogénétique induite par PTU, probablement via la réparation des dommages oxydatifs et la régulation de la voie de signalisation PERK/EIF2α/ATF4 du stress du réticulum endoplasmique.

mots-clés

Shugan Bushen Yulin Tang; propylthiouracile; stress du réticulum endoplasmique; kinase PERK/facteur d'initiation eIF2α/activateur de transcription 4 (ATF4); qualité du sperme

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