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Comparison of Wild and Cultivated Alpiniae Oxyphyllae Fructus Based on Traditional Quality Evaluation

  • role： First author第一作者
  • Affiliation：

    Pharmaceutical College，Harbin University of Commerce，Harbin 150076，China

    Hainan Provincial Key Laboratory of Resources Conservation and Development of Southern Medicine，International Joint Research Center for Traditional Chinese Medicine Quality，Hainan Branch of Institute of Medicinal Plant Development，Chinese Academy of Medical Sciences&Peking Union Medical College，Haikou 570311，China

  • Email：2307375902@qq.com
  • Introduction：王凤帆，在读硕士，从事中药质量标准研究，E-mail：2307375902@qq.com
WANG Fengfan12,  
  • Affiliation：

    Pharmaceutical College，Harbin University of Commerce，Harbin 150076，China

    Hainan Provincial Key Laboratory of Resources Conservation and Development of Southern Medicine，International Joint Research Center for Traditional Chinese Medicine Quality，Hainan Branch of Institute of Medicinal Plant Development，Chinese Academy of Medical Sciences&Peking Union Medical College，Haikou 570311，China

XIANG Yajie12,  
  • Affiliation：

    Hainan Provincial Key Laboratory of Resources Conservation and Development of Southern Medicine，International Joint Research Center for Traditional Chinese Medicine Quality，Hainan Branch of Institute of Medicinal Plant Development，Chinese Academy of Medical Sciences&Peking Union Medical College，Haikou 570311，China

FENG Jian2,  
  • Affiliation：

    Hainan Provincial Key Laboratory of Resources Conservation and Development of Southern Medicine，International Joint Research Center for Traditional Chinese Medicine Quality，Hainan Branch of Institute of Medicinal Plant Development，Chinese Academy of Medical Sciences&Peking Union Medical College，Haikou 570311，China

HOU Wencheng2,  
  • role： Corresponding author通信作者
  • Affiliation：

    Pharmaceutical College，Harbin University of Commerce，Harbin 150076，China

  • Email：lwldzd@163.com
  • Introduction：李文兰，博士，教授，从事中药药效物质基础研究，Tel：0451-84605022，E-mail：lwldzd@163.com； *
LI Wenlan1*,  
  • role： Corresponding author通信作者
  • Affiliation：

    Hainan Provincial Key Laboratory of Resources Conservation and Development of Southern Medicine，International Joint Research Center for Traditional Chinese Medicine Quality，Hainan Branch of Institute of Medicinal Plant Development，Chinese Academy of Medical Sciences&Peking Union Medical College，Haikou 570311，China

  • Email：yyliu@implad.ac.cn
  • Introduction：刘洋洋，博士，研究员，从事中药质量控制技术及其应用研究，Tel：0898-31589011，E-mail：yyliu@implad.ac.cn
LIU Yangyang2*

résumé

ObjectiveTo compare the differences between wild Alpiniae Oxyphyllae Fructus（WAOF） and cultivated Alpiniae Oxyphyllae Fructus（CAOF） through a traditional quality evaluation system for medicinal materials.MethodsA total of 10 batches of WAOF and 12 batches of CAOF samples were collected from various regions of Hainan province. Relevant analytical methods from the 2020 edition of the Pharmacopoeia of the People's Republic of China were employed to observe the characteristics of WAOF and CAOF， followed by microscopic identification， thin-layer chromatography（TLC） identification， moisture content（toluene method）， total ash， acid-insoluble ash， water-soluble and alcohol-soluble extracts（hot dipping method）， water-soluble protein， total polysaccharides and total flavonoids（ultraviolet spectrophotometry）， and volatile oil content（method A under general rule 2204）. The contents of five active components（protocatechuic acid， chrysin， kaempferol， tectochrysin and nootkatone） were quantified using ultra-performance liquid chromatography（UPLC）， and the antioxidant activity was evaluated. Building upon traditional quality evaluation of AOF， quantitative measurements were conducted on its appearance traits including diameter， length， plumpness（diameter/length ratio）， and color. Canonical correlation analysis was performed using SPSS 26.0 to explore relationships between appearance traits and intrinsic quality.ResultsNo significant differences were observed between WAOF and CAOF in microscopic observation， TLC identification， moisture content， protocatechuic acid content， kaempferol content， odor， or antioxidant activity measured by 2，2ʹ-azino-bis（3-ethylbenzothiazoline-6-sulfonic acid）（ABTS） method. WAOF exhibited significantly higher levels in water-soluble extracts， alcohol-soluble extracts， total polysaccharide content， water-soluble protein content， 100-grain weight， length， and total color difference（ΔE*ab） compared to CAOF（P<0.01）. In contrast， CAOF showed significantly higher levels of total ash， acid-insoluble ash， content of total flavonoids， volatile oil content， chrysin content， tectochrysin content， nootkatone content， diameter， plumpness， lightness（L*）， red-green chromaticity（a*）， yellow-blue chromaticity（b*）， and antioxidant activity measured by 1，1-diphenyl-2-picrylhydrazyl（DPPH） method compared to WAOF（P<0.01）. Correlation analysis between 7 phenotypic traits and 8 quality traits revealed that among the phenotypic traits， plumpness， L*， a*， and b* exerted significant influence on intrinsic quality. Among the quality traits， total flavonoids， volatile oils， nootkatone， chrysin， and tectochrysin contributed substantially to intrinsic quality.ConclusionPlumpness， L*， a*， and b* of AOF significantly influence its intrinsic quality， and higher values of these parameters indicate relatively superior intrinsic quality. The comprehensive quality evaluation reveals that CAOF samples collected in this study are superior to their wild counterparts.

mots-clés

Alpiniae Oxyphyllae Fructus;wild;cultivated;traditional quality evaluation;antioxidant;phenotypic traits;quality traits;homology of medicine and food

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