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古典名方血府逐瘀湯の物質基盤に関するLC-MS分析

  • role： First author第一作者
  • Affiliation：

    Institute of Basic Medical Sciences of Xiyuan Hospital，China Academy of Chinese Medical Sciences，Beijing 100091，China

  • Email：zshuj28@163.com
  • Introduction：钟舒静，在读硕士，从事中药复方物质基础研究，E-mail：zshuj28@163.com
ZHONG Shujing,  
  • Affiliation：

    Institute of Basic Medical Sciences of Xiyuan Hospital，China Academy of Chinese Medical Sciences，Beijing 100091，China

LIU Zhaoyue,  
  • Affiliation：

    Institute of Basic Medical Sciences of Xiyuan Hospital，China Academy of Chinese Medical Sciences，Beijing 100091，China

WANG Mengge,  
  • Affiliation：

    Institute of Basic Medical Sciences of Xiyuan Hospital，China Academy of Chinese Medical Sciences，Beijing 100091，China

MIAO Lan,  
  • Affiliation：

    Institute of Basic Medical Sciences of Xiyuan Hospital，China Academy of Chinese Medical Sciences，Beijing 100091，China

REN Changying,  
  • role： Corresponding author通信作者
  • Affiliation：

    Institute of Basic Medical Sciences of Xiyuan Hospital，China Academy of Chinese Medical Sciences，Beijing 100091，China

  • Email：mingqian_sun@163.com
  • Introduction：孙明谦，博士，研究员，从事中医药代谢组学研究，Tel：010-62835618，E-mail：mingqian_sun@163.com
SUN Mingqian*,  
  • role： Corresponding author通信作者
  • Affiliation：

    Institute of Basic Medical Sciences of Xiyuan Hospital，China Academy of Chinese Medical Sciences，Beijing 100091，China

  • Email：tcmlinli@163.com
  • Introduction：林力，博士，研究员，从事中药复方物质基础研究和中药新药研究，Tel：010-62835618，E-mail：tcmlinli@163.com；
LIN Li*

サマリー

本研究の目的は、古典的な処方である血府逐瘀湯の化学成分を体系的に同定し、主要成分の定量分析を行い、その薬効物質基盤を解明し、品質管理基準の改善に科学的根拠を提供することである。超高性能液体クロマトグラフィー・四重極飛行時間型質量分析計（UPLC-Q-TOF-MS/MS）を用いて血府逐瘀湯の化合物を定性分析し、化合物の保持時間、二次質量スペクトルの断片イオン情報、52種類の対照品及びデータベースと比較して化合物を同定し、化合物の由来を帰属付けた。代表的な22種類の化合物を選定し、超高性能液体クロマトグラフィー・四重極線形イオントラップ質量分析計（UPLC-Q-TRAP-MS/MS）で複合成分の定量分析を行った。結果として血府逐瘀湯から計77種類の化合物を同定し、その内訳はフラボノイド類31種で主に枳殻、甘草、桃仁、紅花、柴胡、赤芍、牛膝由来；トリテルペン類24種は桔梗、甘草、赤芍、地黄由来；フェニルプロパノイド類及び誘導体9種は川芎、当帰、地黄由来；フェノール酸類4種は川芎、当帰、赤芍由来；糖類3種は地黄、牛膝由来；その他6種は桃仁、地黄、当帰由来であった。定量分析により、血府逐瘀湯中の原カテキン酸、ヒドロキシベニシクレマノイド、苦杏仁配糖体、バニリン酸、シャクヤク配糖体、セリトログリコシド、グリチルリチン、イソクエルシトリン、ユズリュウ苷、コスモス苷、オレンジ配糖体、新オレンジ配糖体、イソグリチルリチン、グリチル素、ユズリュウ素、ベンゾイルシャクヤク配糖体、オレンジ素、イソグリチル素、マングリン花素、グリチル酸、川陳皮素、ゴウホンナイリツの質量分率がそれぞれ0.12、1.57、54.53、0.29、36.17、4.29、4.84、0.09、46.67、0.04、3.44、31.95、0.82、0.10、0.11、0.43、0.07、0.03、0.01、8.24、0.13、1.81 mg·g-1であることが測定された。結論として、本研究で確立した定性分析法は血府逐瘀湯の化学成分を迅速かつ高感度に分析でき、52種類の化合物が対照品により確認されており十分な精度を保証し、22種の成分について定量分析を行い、本方剤の薬効物質基盤研究と品質管理基準向上のためのデータ支援を提供する。

キーワード

血府逐瘀湯;超高性能液体クロマトグラフィー-四重極-飛行時間質量分析法（UPLC-Q-TOF-MS/MS）;超高性能液体クロマトグラフィー-四重極-線形イオントラップ質量分析法（UPLC-Q-TRAP-MS/MS）;古典名方;漢方薬;定性分析;定量分析

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