이전|다음

고전 명방 혈부축어탕 물질 기초의 LC-MS 분석

  • role： First author第一作者
  • Affiliation：

    Institute of Basic Medical Sciences of Xiyuan Hospital，China Academy of Chinese Medical Sciences，Beijing 100091，China

  • Email：zshuj28@163.com
  • Introduction：钟舒静，在读硕士，从事中药复方物质基础研究，E-mail：zshuj28@163.com
ZHONG Shujing,  
  • Affiliation：

    Institute of Basic Medical Sciences of Xiyuan Hospital，China Academy of Chinese Medical Sciences，Beijing 100091，China

LIU Zhaoyue,  
  • Affiliation：

    Institute of Basic Medical Sciences of Xiyuan Hospital，China Academy of Chinese Medical Sciences，Beijing 100091，China

WANG Mengge,  
  • Affiliation：

    Institute of Basic Medical Sciences of Xiyuan Hospital，China Academy of Chinese Medical Sciences，Beijing 100091，China

MIAO Lan,  
  • Affiliation：

    Institute of Basic Medical Sciences of Xiyuan Hospital，China Academy of Chinese Medical Sciences，Beijing 100091，China

REN Changying,  
  • role： Corresponding author通信作者
  • Affiliation：

    Institute of Basic Medical Sciences of Xiyuan Hospital，China Academy of Chinese Medical Sciences，Beijing 100091，China

  • Email：mingqian_sun@163.com
  • Introduction：孙明谦，博士，研究员，从事中医药代谢组学研究，Tel：010-62835618，E-mail：mingqian_sun@163.com
SUN Mingqian*,  
  • role： Corresponding author通信作者
  • Affiliation：

    Institute of Basic Medical Sciences of Xiyuan Hospital，China Academy of Chinese Medical Sciences，Beijing 100091，China

  • Email：tcmlinli@163.com
  • Introduction：林力，博士，研究员，从事中药复方物质基础研究和中药新药研究，Tel：010-62835618，E-mail：tcmlinli@163.com；
LIN Li*

추상적인

본 연구의 목적은 고전 명방 혈부축어탕의 화학 성분을 체계적으로 동정하고 주요 성분에 대해 정량 분석하여 약효 물질 기초를 밝히며 품질 관리 기준 개선을 위한 과학적 근거를 제공하는 것이다. 초고성능 액체 크로마토그래피-사중극자-비행시간 질량분석법(UPLC-Q-TOF-MS/MS)을 이용하여 혈부축어탕의 화합물을 정성 분석하였고, 화합물의 보유 시간, 2차 질량 스펙트럼 파편 이온 정보, 52종 대조품 및 데이터베이스와 비교하여 화합물을 확정하고 출처를 분류하였다. 대표성 화합물 22종을 선별하여 초고성능 액체 크로마토그래피-사중극자-선형 이온 함정 질량분석법(UPLC-Q-TRAP-MS/MS)으로 복합성분을 정량 분석하였다. 혈부축어탕에서 총 77종의 화합물이 확인되었으며, 플라보노이드류 31종은 주로 지각, 감초, 도인, 홍화, 시호, 적작, 우슬에서 유래하였고, 테르펜류 24종은 길경, 감초, 적작, 지황에서, 벤질프로파노이드류 및 유도체 9종은 천궁, 당귀, 지황에서, 페놀산류 4종은 천궁, 당귀, 적작에서, 당류 3종은 지황, 우슬에서, 기타 6종은 도인, 지황, 당귀에서 유래하였다. 정량 분석 결과 혈부축어탕 내 원 카테킨산, 하이드록시 쇼가요글리코사이드, 쓴살구 배당체, 바닐산, 작약 배당체, 감초 당 배당체, 감초 배당체, 이소퀘르세틴, 유피린, 코스메틴 배당체, 오렌지 배당체, 신 오렌지 배당체, 이소감초 배당체, 감초 성분, 유피린, 벤조일 작약 배당체, 오렌지 성분, 이소감초 성분, 망병화소, 감초 산, 천 진피 성분, 고본내륜의 질량 분율이 각각 0.12, 1.57, 54.53, 0.29, 36.17, 4.29, 4.84, 0.09, 46.67, 0.04, 3.44, 31.95, 0.82, 0.10, 0.11, 0.43, 0.07, 0.03, 0.01, 8.24, 0.13, 1.81 mg·g-1로 측정되었다. 결론적으로 본 연구에서 확립한 정성 방법은 혈부축어탕의 화학 성분을 신속하고 민감하게 분석할 수 있으며, 52종의 화합물이 대조품을 통해 확증되어 충분한 정확성을 보장하고, 22종 성분에 대해 정량 분석을 수행하여 본 처방의 약효 물질 기초 연구와 품질 관리 기준 향상에 데이터 지원을 제공한다.

키워드

혈부축어탕;초고성능 액체 크로마토그래피-사중극자-비행시간 질량분석법(UPLC-Q-TOF-MS/MS);초고성능 액체 크로마토그래피-사중극자-선형 이온 함정 질량분석법(UPLC-Q-TRAP-MS/MS);고전 명방;중의학;정성 분석;정량 분석

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