The blood-brain barrier (BBB) as a brain capillary wall and glial cells in the form of plasma and the barrier between the brain cells and formed by choroid plexus barrier between the plasma and cerebrospinal fluid, (mostly) of harmful substances that may prevent some brain tissue, so as to keep within the brain tissue environment basically stable, but at the same time its stop most drugs into the intracranial, It brings difficulties to the treatment of craniocerebral diseases. The establishment of blood-brain barrier experimental model is a key technique for drug therapy of brain diseases.