Objective: To provide guidance for designing sodium/glucose co-transporters-2(SGLT-2)inhibitors. Method: We collected lots of active micro-molecules of sodium/glucose co-transporters-2 (SGLT-2) inhibitors from relevant literatures, and screened out 24 micro-molecules to build a training set, and obtained 3D-pharmacophore of SGLT-2 inhibitors by using HypoGen method with Discover Studio 3.0. Result: The best pharmacophore that we generated contained one hydrogen-bonding donor, one hydrogen-bonding acceptor, one hydrophobic grouping and five hydrophobic groups. The model was verified by Fischer' s randomization and decoy set for its eligibility. Conclusion: SGLT-2 protein played an important role in maintaining blood sugar balance. In recent years, more and more researchers have paid much attention to the protein as a target for treating diabetes. This method was used to successfully build the pharmacophore of SGLT-2 inhibitors, so as to provide guidance for subsequent studies.

SGLT-2 inhibitor;pharmacophore;Fischer's randomization;decoy set verification