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Estudio del mecanismo de acción del alcaloide de raíz medicinal en el tratamiento de la colitis ulcerosa en ratones basado en metabolómica

  • role： First author第一作者
  • Affiliation：

    The Fifth Medical Center of Chinese PLA General Hospital，Beijing 100039，China

    Chinese PLA Medical School， Beijing 100853，China

  • Email：shengqi6868@163.com
  • Introduction：牛圣奇，在读硕士，从事中药药理学研究及中药新药研发研究，E-mail：shengqi6868@163.com
NIU Shengqi12,  
  • Affiliation：

    The Fifth Medical Center of Chinese PLA General Hospital，Beijing 100039，China

LANG Liwei1,  
  • Affiliation：

    The Fifth Medical Center of Chinese PLA General Hospital，Beijing 100039，China

LI Xing1,  
  • Affiliation：

    The Fifth Medical Center of Chinese PLA General Hospital，Beijing 100039，China

LI Haotian1,  
  • Affiliation：

    The Fifth Medical Center of Chinese PLA General Hospital，Beijing 100039，China

WEI Shizhang1,  
  • Affiliation：

    The Fifth Medical Center of Chinese PLA General Hospital，Beijing 100039，China

JING Manyi1,  
  • role： Corresponding author通信作者
  • Affiliation：

    The Fifth Medical Center of Chinese PLA General Hospital，Beijing 100039，China

    Chinese PLA Medical School， Beijing 100853，China

  • Email：zhaoyl2855@126.com
  • Introduction：赵艳玲，研究员，博士生导师，从事中药药理学及中药新药研发，E-mail：zhaoyl2855@126.com
ZHAO Yanling12*

resumen

El objetivo es investigar el efecto del alcaloide de raíz medicinal sobre los metabolitos endógenos y las vías metabólicas en ratones con colitis ulcerosa. Método: utilizando un modelo de enfermedad de colitis ulcerosa en ratones inducido por 3 % de dextrano sulfato de sodio (DSS), se administraron diferentes dosis orales del alcaloide de raíz medicinal a los ratones. Se observó el efecto terapéutico mediante el peso corporal, la longitud del colon, el índice de actividad de la enfermedad (DAI) y la histopatología del tejido del colon; mediante cromatografía líquida ultraeficiente acoplada a espectrometría de masas de tiempo de vuelo con cuadrupolo (UPLC-Q-TOF/MS) se midieron los niveles de metabolitos en suero y heces de los ratones, combinando métodos metabolómicos para seleccionar metabolitos diferenciales y examinar el posible mecanismo terapéutico del alcaloide en el tratamiento de la colitis ulcerosa inducida por DSS en ratones. Resultados: tras la intervención con el alcaloide, comparado con el grupo modelo, el DAI de los ratones disminuyó significativamente, la longitud del colon se restauró y la histopatología del colon mejoró de manera notable. El estudio metabolómico mostró 13 metabolitos diferenciales en el suero y 8 en las heces, y el análisis de enriquecimiento de vías identificó 3 rutas metabólicas principales potenciales: biosíntesis de ácidos grasos insaturados, biosíntesis de fenilalanina, tirosina y triptófano, así como metabolismo de fenilalanina. Conclusión: el alcaloide de raíz medicinal posiblemente ejerce su acción terapéutica en la colitis ulcerosa mediante su participación en rutas metabólicas como la síntesis y metabolismo de aminoácidos y biosíntesis de ácidos grasos insaturados.

palabra clave

Alcaloide de raíz medicinal; colitis ulcerosa; metabolómica; dextrano sulfato de sodio

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