Mecanismo por el cual el propiltiouracilo daña la función espermatogénica y la calidad del esperma en ratas a través del estrés del retículo endoplásmico e intervención de Shugan Bushen Yulin Tang

  • role: First author第一作者
  • Affiliation:

    School of Basic Medical Sciences, Hubei University of Chinese Medicine, Wuhan 430065, China

    Hubei Shizhen Laboratory, Wuhan 430065, China

  • Email:1653600142@qq.com
  • Introduction:孟宇豪,在读硕士,从事方剂配伍与临床应用及中西医结合防治不孕不育研究,E-mail:1653600142@qq.com
MENG Yuhao12,  
  • Affiliation:

    Hubei Shizhen Laboratory, Wuhan 430065, China

    Laboratory Animal Center, Hubei University of Chinese Medicine, Wuhan 430065, China

JIANG Xiaocui23,  
  • role: Corresponding author通信作者
  • Affiliation:

    Hubei Shizhen Laboratory, Wuhan 430065, China

    Laboratory Animal Center, Hubei University of Chinese Medicine, Wuhan 430065, China

  • Email:531637551@qq.com
  • Introduction:萧闵,博士,副研究员,从事动物模型及中西医结合防治不孕不育研究,E-mail:531637551@qq.com
XIAO Min23*,  
  • role: Corresponding author通信作者
  • Affiliation:

    School of Basic Medical Sciences, Hubei University of Chinese Medicine, Wuhan 430065, China

    Hubei Shizhen Laboratory, Wuhan 430065, China

  • Email:43382275@qq.com
  • Introduction:王朝阳,博士,教授,从事中医藏象理论及其临床应用研究,E-mail:43382275@qq.com; *
WANG Chaoyang12*

resumen

El objetivo es investigar el mecanismo mediante el cual la fórmula Shugan Bushen Yulin Tang mejora la disfunción espermatogénica y la reducción de la calidad del esperma inducidas por el propiltiouracilo (PTU) en ratas, basado en la vía del estrés del retículo endoplásmico (ERS). Métodos: 60 ratas macho fueron distribuidas aleatoriamente en grupos control, modelo, dosis baja, media y alta de Shugan Bushen Yulin Tang y grupo carnitina, con 10 ratas en cada grupo. Excepto el grupo control, los demás recibieron PTU por vía oral durante 12 días para modelar la disfunción. Posteriormente, los grupos de tratamiento recibieron sus respectivas dosis durante 28 días. Se evaluaron índices de órganos, cambios histopatológicos, niveles hormonales, estrés oxidativo, apoptosis celular y expresión de proteínas relacionadas con ERS. Resultados mostraron deterioro en el grupo modelo que fue mejorado con los tratamientos. Conclusión: Shugan Bushen Yulin Tang mejora la disfunción espermatogénica inducida por PTU, posiblemente mediante la reparación del daño oxidativo y regulación de la vía PERK/EIF2α/ATF4 del estrés del retículo endoplásmico.

palabra clave

Shugan Bushen Yulin Tang; propiltiouracilo; estrés del retículo endoplásmico; quinasa PERK/factor de inicio de traducción eIF2α/activador de transcripción 4 (ATF4); calidad del esperma

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