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Estudio del mecanismo regulador de la activación de las células gliales en el cerebro de ratones utilizando antidepresivos y posiblemente con la vía JAK2/STAT3

  • role： First author第一作者
  • Affiliation：

    Institute of Basic Theory for Chinese Medicine， China Academy of Chinese Medical Sciences，Beijing 100700， China

  • Email：1620490253@qq.com
  • Introduction：王英，在读硕士，从事情志病的中医防治及方证相关研究，E-mail：1620490253@qq.com
WANG Ying1,  
  • Affiliation：

    Ningxia Chinese Medicine Research Center，Ningxia 750000， China

GONG Zihan2,  
  • Affiliation：

    Hengshanqiao People's Hospital in Changzhou Economic Development Zone， Changzhou 213161， China

LIANG Wenqing3,  
  • Affiliation：

    Institute of Basic Research in Clinical Medicine， China Academy of Chinese Medical Sciences，Beijing 100700， China

YANG Jingwen4,  
  • role： Corresponding author通信作者
  • Affiliation：

    Institute of Basic Theory for Chinese Medicine， China Academy of Chinese Medical Sciences，Beijing 100700， China

  • Email：yuegx73@hotmail.com
  • Introduction：岳广欣，博士，研究员，从事情志病的中医防治及方证相关研究，E-mail：yuegx73@hotmail.com
YUE Guangxin1*

resumen

Este estudio tiene como objetivo investigar el mecanismo de acción de los antidepresivos en la activación de células gliales en el cerebro de ratones después de la separación de la madre y la descendencia y el doble impacto. En comparación con el grupo NC, los ratones del grupo MRS mostraron comportamiento depresivo, los niveles de neurotransmisores en el hipocampo disminuyeron significativamente (P<0.05, P<0.01), la proteína Iba-1 aumentó (P<0.01), los niveles de proteínas JAK2 y STAT3 aumentaron (P<0.05), los CD68, CD11b, IL-1β, JAK2, STAT3 mRNA aumentaron significativamente (P<0.01), los niveles de IL-10 mRNA disminuyeron significativamente (P<0.01). En comparación con el grupo MRS, los grupos XSF y minociclina mostraron una mejora en el comportamiento depresivo, el contenido de neurotransmisores en el hipocampo de los ratones de los grupos XSF y minociclina aumentó significativamente (P<0.05, P<0.01), el nivel de Iba-1 disminuyó significativamente (P<0.01), los niveles de proteínas JAK2 y STAT3 disminuyeron (P<0.05), los niveles de mRNA de CD68, CD11b, JAK2, STAT3 y IL-1β disminuyeron significativamente (P<0.05, P<0.01), los niveles de mRNA de IL-10 aumentaron significativamente (P<0.05, P<0.01). Conclusión: Los antidepresivos pueden aliviar el comportamiento depresivo de los ratones expuestos a la separación de la madre y la descendencia después de un doble impacto, y es posible que el mecanismo de alivio de la activación de las células gliales del hipocampo por los antidepresivos esté relacionado con la vía JAK2/STAT3.

palabra clave

separación madre-hijo; comportamiento depresivo; células gliales; vía JAK2/STAT3; antidepresivo

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