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Estudio del impacto del medicamento para la desintoxicación y la humectación en la transdiferenciación de las células TCMK-1 a través de la vía SIRT1/FoxO1

  • role： First author第一作者
  • Affiliation：

    The Eighth Clinical Medical College of Guangzhou University of Chinese Medicine，Foshan 528000，China

  • Email：15732036181@163.com
  • Introduction：李清茹，博士，医师，从事中医药防治肾脏病研究，E-mail：15732036181@163.com
LI Qingru1,  
  • role： Corresponding author通信作者
  • Affiliation：

    The First Affiliated Hospital of Henan University of Chinese Medicine，Zhengzhou 450000，China

  • Email：zlq971688@126.com
  • Introduction：张琳琪，博士，主任医师，从事中医药防治肾脏病研究，E-mail：zlq971688@126.com；
ZHANG Linqi2*,  
  • role： Corresponding author通信作者
  • Affiliation：

    Shenzhen Bao'an Authentic Traditional Chinese Medicine Therapy Hospital，Shenzhen 518100，China

  • Email：835492967@qq.com
  • Introduction：黎斌怡，硕士，主治医师，从事中医药防治肿瘤研究，E-mail：835492967@qq.com
LI Binyi3*,  
  • Affiliation：

    The First Affiliated Hospital of Henan University of Chinese Medicine，Zhengzhou 450000，China

GE Zihao2

resumen

El objetivo de la investigación fue explorar el efecto protector de la transdiferenciación de las células epiteliales del túbulo renal TCMK-1 inducida por el factor de crecimiento beta-β 1 (TGF-β 1) y las vías potenciales de acción del medicamento para la desintoxicación y humectación de los riñones. Los métodos utilizaron una concentración masiva de 10 μg·L-1 de TGF-β 1 para inducir la transdifferentiation de las células TCMK-1, con la creación de los siguientes grupos: grupos en blanco, grupos modelo, grupos de medicamentos para la desintoxicación y humectación (10% de suero de medicamentos para la desintoxicación y humectación), grupos de losartán (10% de suero de losartán), grupos EX527 + TGF-β 1, grupos EX527+ medicamento para la desintoxicación y humectación, grupos EX527.. Las pruebas de fluorescencia inmunológica se utilizaron para probar la expresión de la actina α-lisa (actina α-lisa), de la cadherina E-calcio (E-cadherina), de la proteína ligera 3 de la cadena 1 de microtúbulos (LC3) y de la autopuesta 1 (Atg6) en la localización de las proteínas, así como para probar los niveles de expresión de los medicamentos de losartán, FoxO1 (Ac-FoxO1). Las tasas de cambio de las proteínas se determinaron mediante pruebas de inmunotransferencia (Western blot) y reacción en cadena de la polimerasa en tiempo real (PCR en tiempo real), con un aumento significativo de la expresión de la actina α-lisa y AC-FoxO1 en los grupos de modelo, los grupos de losartán

palabra clave

Medicamento para la desintoxicación y la humectación; células del túbulo renal (TCMK-1); vía de inducción de la información 1 (SIRT1)/marco proteico histona 1 (FoxO1); transdiferenciación; autofagia

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