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Effect of Tongmai Kaiqiao Pills on Mitochondrial Biogenesis of Hippocampal Neurons in Rats with Vascular Cognitive Impairment Based on AMPK/PGC-1α Signaling Pathway

  • role： First author第一作者
  • Affiliation：

    School of Rehabilitation Medicine，Henan University of Chinese Medicine，Zhengzhou 450046，China

  • Email：819931585@qq.com
  • Introduction：马璐瑶，在读硕士，从事神经系统疾病损伤后功能障碍的康复治疗研究，E-mail：819931585@qq.com
MA Luyao1,  
  • role： Corresponding author通信作者
  • Affiliation：

    Henan Province Hospital of Traditional Chinese Medicine，Zhengzhou 450053，China

  • Email：yanjieli2008@126.com
  • Introduction：李彦杰，硕士，主任医师，硕士生导师，从事中西医结合治疗神经系统疾病研究，Tel：0371-60908747，E-mail：yanjieli2008@126.com
LI Yanjie2*,  
  • Affiliation：

    School of Rehabilitation Medicine，Henan University of Chinese Medicine，Zhengzhou 450046，China

LIU Haoyuan1,  
  • Affiliation：

    The First Affiliated Hospital of Henan University of Chinese Medicine，Zhengzhou 450003，China

BAI Yanjie3,  
  • Affiliation：

    Henan Province Hospital of Traditional Chinese Medicine，Zhengzhou 450053，China

XING Ruoxing2

resumen

El objetivo de la investigación es observar el efecto de las tabletas de desbloqueo de las vías arteriales en la activación de la proteína AMP (AMPK) / activador subunitario γ PGC-1α (PGC-1α) y el efecto sobre la biogénesis mitocondrial, para estudiar el mecanismo potencial de mejora del déficit cognitivo en ratas con infarto cerebral (VCI) y acelerar su mejora. Se seleccionaron 12 ratas machos de entre 72 ratas, 12 de ellas fueron elegidas para someterse a una operación ficticia y las ratas restantes se sometieron a una operación mejorada a 2VO; las ratas exitosas fueron divididas en un grupo modelo, un grupo de dosis alta y un grupo de dosis baja (27.6 y 13.8 g / kg), un grupo combinado (27.6 g / kg de tabletas para desbloqueo de vías + Dorsomorfina 25 mg / kg), con 4 semanas de inyecciones intraabdominales repetidas del medicamento apropiado, utilizando la prueba del laberinto acuático de Morris para probar la capacidad de aprendizaje y memoria de las ratas. Se utilizó una tinción especial en hematoxilina y eosina (HE) para evaluar los cambios histológicos en el hipocampo de las ratas; se utilizó un método de dosificación de trifosfato de adenosina (ATP) para probar los tejidos cerebrales. El nivel de oxígeno activo celular en los tejidos cerebrales fue evaluado utilizando la prueba MitoSOX Red para detectar el nivel de oxígeno activo celular (ROS) en las ratas; el número de copias de ADN mitocondrial (mtDNA) fue probado utilizando la reacción en cadena de la polimerasa en tiempo real (PCR en tiempo real) con precisión cuántica de fluorescencia; se utilizó la microscopía electrónica de transmisión (TEM) para observar los cambios patológicos de las mitocondrias; el nivel de proteínas fue probado utilizando una prueba de inmunotintado (Western blot) en las ratas en los tejidos cerebrales para evaluar las cantidades de proteínas AMPK y PGC-1α

palabra clave

Tongmai Kaiqiao pills;vascular cognitive impairment;mitochondrial biogenesis;AMP-activated protein kinase （AMPK）/peroxisome proliferator-activated receptor gamma coactivator 1α （PGC-1α） signaling pathway

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