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Identificación de los componentes in vivo e in vitro del brebaje Dan Shen basado en UPLC-Q-TOF-MS/MS

  • role： First author第一作者
  • Affiliation：

    Key Laboratory for Standardization of Chinese Medicines，Ministry of Education， Shanghai Key Laboratory for Compound Chinese Medicines，Institute of Chinese Materia Medica， Shanghai University of Traditional Chinese Medicine，Shanghai 201203，China

  • Email：zhang.st@outlook.com
  • Introduction：张思彤，在读硕士，从事中药药效物质基础与体内过程研究，E-mail：zhang.st@outlook.com
ZHANG Sitong,  
  • Affiliation：

    Key Laboratory for Standardization of Chinese Medicines，Ministry of Education， Shanghai Key Laboratory for Compound Chinese Medicines，Institute of Chinese Materia Medica， Shanghai University of Traditional Chinese Medicine，Shanghai 201203，China

HU Xianrun,  
  • Affiliation：

    Key Laboratory for Standardization of Chinese Medicines，Ministry of Education， Shanghai Key Laboratory for Compound Chinese Medicines，Institute of Chinese Materia Medica， Shanghai University of Traditional Chinese Medicine，Shanghai 201203，China

LIU Wenkang,  
  • Affiliation：

    Key Laboratory for Standardization of Chinese Medicines，Ministry of Education， Shanghai Key Laboratory for Compound Chinese Medicines，Institute of Chinese Materia Medica， Shanghai University of Traditional Chinese Medicine，Shanghai 201203，China

LEI Jinchun,  
  • Affiliation：

    Key Laboratory for Standardization of Chinese Medicines，Ministry of Education， Shanghai Key Laboratory for Compound Chinese Medicines，Institute of Chinese Materia Medica， Shanghai University of Traditional Chinese Medicine，Shanghai 201203，China

CHENG Xuemei,  
  • Affiliation：

    Key Laboratory for Standardization of Chinese Medicines，Ministry of Education， Shanghai Key Laboratory for Compound Chinese Medicines，Institute of Chinese Materia Medica， Shanghai University of Traditional Chinese Medicine，Shanghai 201203，China

WU Xiaojun,  
  • Affiliation：

    Key Laboratory for Standardization of Chinese Medicines，Ministry of Education， Shanghai Key Laboratory for Compound Chinese Medicines，Institute of Chinese Materia Medica， Shanghai University of Traditional Chinese Medicine，Shanghai 201203，China

CHEN Wansheng,  
  • role： Corresponding author通信作者
  • Affiliation：

    Key Laboratory for Standardization of Chinese Medicines，Ministry of Education， Shanghai Key Laboratory for Compound Chinese Medicines，Institute of Chinese Materia Medica， Shanghai University of Traditional Chinese Medicine，Shanghai 201203，China

  • Email：manlinli_cn@163.com
  • Introduction：李曼琳，博士，助理研究员，从事中药药效物质基础与药物代谢动力学研究，Tel：021-51322504，E-mail：manlinli_cn@163.com； *
LI Manlin*,  
  • role： Corresponding author通信作者
  • Affiliation：

    Key Laboratory for Standardization of Chinese Medicines，Ministry of Education， Shanghai Key Laboratory for Compound Chinese Medicines，Institute of Chinese Materia Medica， Shanghai University of Traditional Chinese Medicine，Shanghai 201203，China

  • Email：wchcxm@163.com
  • Introduction：王长虹，博士，研究员，博士生导师，从事新药制剂与体内过程研究，Tel：021-51322511，E-mail：wchcxm@163.com
WANG Changhong*

resumen

El objetivo es aclarar la composición química del brebaje Dan Shen y los componentes sanguíneos después de la administración oral en ratas. El método utiliza cromatografía líquida ultra eficiente acoplada a espectrometría de masas de tiempo de vuelo y cuadrupolo en tándem (UPLC-Q-TOF-MS/MS), combinada con el software PeakView 1.2. Basándose en el tiempo de retención, el pico de ion quasi-molecular, los fragmentos secundarios y la comparación con la literatura, se llevó a cabo la caracterización e identificación sistemática de los componentes del extracto acuoso del brebaje Dan Shen y los componentes migrados en la sangre de las ratas después de la administración oral. Además, se identificaron los componentes químicos del extracto acuoso de Dan Shen, sándalo y amomo cocidos conjuntamente para aclarar el origen de los componentes químicos en el extracto acuoso del brebaje Dan Shen. Resultados: se identificaron en total 73 compuestos, incluyendo 21 ácidos fenólicos, 23 diterpenos, 6 flavonoides, 7 ácidos orgánicos, 3 aceites volátiles y 13 otros tipos, a partir del extracto acuoso del brebaje Dan Shen. En el plasma sanguíneo de ratas con medicación se identificaron 36 componentes originales en la sangre y 15 metabolitos, con vías metabólicas principales que incluyen reducción, hidratación, hidroxilación, desmetilación, metilación, sulfatación, etc. Estos metabolitos principalmente provienen de los componentes diterpénicos y ácidos fenólicos de Dan Shen. Conclusión: los componentes sanguíneos del extracto acuoso del brebaje Dan Shen son principalmente ácidos fenólicos y diterpenos, lo que puede ser la base material de su efecto farmacológico, y puede proporcionar referencia para la investigación farmacológica, control de calidad y aplicación clínica del brebaje Dan Shen.

palabra clave

brebaje Dan Shen; componentes químicos; química de fármacos séricos; metabolitos; reglas de fragmentación; cromatografía líquida ultrarrápida - cuadrupolo - espectrometría de masas en tándem de tiempo de vuelo (UPLC-Q-TOF-MS/MS)

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