Determination of Chlorogenic Acid in Flos Lonicerae and“Liyan Zhike Chongji”by HPLC
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Determination of Chlorogenic Acid in Flos Lonicerae and“Liyan Zhike Chongji”by HPLC
Chinese Journal of Experimental Traditional Medical FormulaeVol. 2, Issue 5, Pages: 5-7(1996)
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CLC:R286
Published:1996
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Zhu Qing, Li Guanghui, Hou Xiaoming, et al. Determination of Chlorogenic Acid in Flos Lonicerae and“Liyan Zhike Chongji”by HPLC[J]. Chinese journal of experimental traditional medical formulae, 1996, 2(5): 5-7.
DOI:
Zhu Qing, Li Guanghui, Hou Xiaoming, et al. Determination of Chlorogenic Acid in Flos Lonicerae and“Liyan Zhike Chongji”by HPLC[J]. Chinese journal of experimental traditional medical formulae, 1996, 2(5): 5-7.DOI:
Determination of Chlorogenic Acid in Flos Lonicerae and“Liyan Zhike Chongji”by HPLC
Chlorogenic acid in Flos lonicerae and“Li Yan Zhi Ke Chong Ji”was determinated by high performance liquid chromatography.Chlorogenic acid was separated on KYWG-C18 column and detected at 323nm
using water-acetonitrile-acetic acid(110:3:3)as mobile phase. There is a good linearity from 0.01 to 0.10 mg/ml of gatrodin(r=0.9998
Y=-2.4605×103+5.6138x105X). The average recovery was 100.87% and the relative standard deviation was 1.55%(n=6).This method is simple