Determination of the Pueraria Content in“Xinanning Pian” by RP-HPLC
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Determination of the Pueraria Content in“Xinanning Pian” by RP-HPLC
Chinese Journal of Experimental Traditional Medical FormulaeVol. 2, Issue 5, Pages: 2-5(1996)
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CLC:R286
Published:1996
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Tan Shengjian1, Chen Peirang1, Gao Ye1, et al. Determination of the Pueraria Content in“Xinanning Pian” by RP-HPLC[J]. Chinese journal of experimental traditional medical formulae, 1996, 2(5): 2-5.
DOI:
Tan Shengjian1, Chen Peirang1, Gao Ye1, et al. Determination of the Pueraria Content in“Xinanning Pian” by RP-HPLC[J]. Chinese journal of experimental traditional medical formulae, 1996, 2(5): 2-5.DOI:
Determination of the Pueraria Content in“Xinanning Pian” by RP-HPLC
A quantitative metbd for the determination oh the pueraria content in Xinanning Pian was established. The sampe was extracted with alcohol by reflux in a water bath. The resolution of pueraria and the adjacent peak was 1.5 as determined by RP-HPLC. The number of theoretical plates calculated by pueraria peak was 1510.The standard curve was linear in the range of 0.264~2.640 μg and the correlation coefficient was 0.9999.The average recovery was 100.7%. The method is simple and accurate and thus is satisfactory for the determianation of the pueraria content in Xinanning Pian