Using the model mice with spleen-deficiency syndrome induced by rhubarb

the IL-2 activity was detected by the method of incorporation of tritiated thymidine

NK activity by the method of tritiated thymidine labeled target cells and IFN-γ-titer by the method of micro-cytopathic effects. The NK activity

IL-2 and IFN-γ levels decreased significantly in the spleen-deficiency groups

while those parameters could be improved to normal level by Huangqi Jianzhong Decoction (HJD) and Buzhong Yiqi Decoction (BYD)

there was no significant change in normal groups treated with HJD or BYD.