Objective:To develop a HPLC method for determination of paeoniflorin and limit examination of aconitine in compound Xiaohuoluo pills.Method: For paeoniflorin

C18 column（4.6 mm×150 mm

5 μm） was used at 30 ℃;with the mobile phase was acetonitrile-0.4% phosphate solution（14∶86）.The detection wavelength was 230 nm and the flow rate wsa set at 1.0 mL · min-1.For aconitine

C18 column（4.6 mm×150 mm

5 μm） was used at 35 ℃;with the mobile phase was acetonitrile-tetrahydrofuran（25∶15）and 0.1 mol·L-1 ammonium acetate solution by gradient elution.The detection wavelength was 235 nm and the flow rate wsa set at 0.8 mL · min-1.Result: The linear range of Paeoniflorin was 0.035 5-0.709 6 μg（r=0.999 9）.The average recoveries was 101.9% and RSD was 1.86%（n=6）.The linear range of aconitine was 0.066 0-0.495 0 μg（r=0.999 8）.The average recoveries was 102.0% and RSD was 1.75%（n=6）.Conclusion: The method is simple

accurate

stable and can be used for determination of paeoniflorin and limit examination of aconitine in compound Xiaohuoluo pills.