WANG Jun-ming1, MIAO Ming-san3, QU Ling-bo1, et al. Status and Proposals of Applying Proteomics to Research Essence of Liver Injury[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(9): 358-362.
WANG Jun-ming1, MIAO Ming-san3, QU Ling-bo1, et al. Status and Proposals of Applying Proteomics to Research Essence of Liver Injury[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(9): 358-362.DOI:
Status and Proposals of Applying Proteomics to Research Essence of Liver Injury
To discuss status and proposals of applying proteomics to research essence of liver injury.Looking through the related literatures from home and abroad
then analyzing and compiling them.①Proteomics technique has been applied to many types of liver injury induced by drug
carbon tetrachloride(CCl4)
alcohol
lipopolysaccharides(LPS)
immune related
thioacetamide
radiation
bromobenzene
hydrazine
or non-alcoholic fatty liver disease(NAFLD).②The protein samples for analysis are mainly taken from the animal liver
serum
urine
bile acids
mitochondria
etc.③Main analyzing tools of proteomics are two-dimensional gel electrophoresis(2-DE)
Matrix-assisted laser desorption/ionization time of flight mass spectrometry(MALDI-TOF-MS) and multi-stage MS.④Research on proteome mechanism of liver injury needs to be strengthened.⑤Hepatoprotective effect of drugs is not enough through proteomics.We should strengthen researches on essence of liver injury and essence-based hepatoprotection of drugs through proteomics.