Determination of Gallic Acid and Quercetin in Pithecellobium Clypearia Antiphlogistic Capsule by HPLC

CHEN Yan-wei1; ZHANG Huan2; YANG Shi-lei1; WANG Chun-fu1; LIANG Hui1

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Determination of Gallic Acid and Quercetin in Pithecellobium Clypearia Antiphlogistic Capsule by HPLC

更新时间：2024-09-23

- Determination of Gallic Acid and Quercetin in Pithecellobium Clypearia Antiphlogistic Capsule by HPLC
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 19, Issue 9, Pages: 126-128(2013)
- 作者机构：
  
  1. 大连医科大学附属第一医院
  2. 大连医科大学药学院
- 作者简介：
- 基金信息：
- DOI：
  CLC： R284.1
- Published：2013
- 稿件说明：
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[1]陈艳伟,张欢,杨世磊,王春富,梁卉.HPLC同时测定猴耳环消炎胶囊中没食子酸与槲皮素的含量[J].中国实验方剂学杂志,2013,19(09):126-128.

CHEN Yan-wei1, ZHANG Huan2, YANG Shi-lei1, et al. Determination of Gallic Acid and Quercetin in Pithecellobium Clypearia Antiphlogistic Capsule by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(9): 126-128.
[1]陈艳伟,张欢,杨世磊,王春富,梁卉.HPLC同时测定猴耳环消炎胶囊中没食子酸与槲皮素的含量[J].中国实验方剂学杂志,2013,19(09):126-128. DOI：

CHEN Yan-wei1, ZHANG Huan2, YANG Shi-lei1, et al. Determination of Gallic Acid and Quercetin in Pithecellobium Clypearia Antiphlogistic Capsule by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(9): 126-128. DOI：

摘要

目的:建立高效液相色谱法同时测定猴耳环消炎胶囊中没食子酸与槲皮素的含量。方法:伊利特Hypersil BDS-C18（4.6 mm×150 mm

5μm）色谱柱进行色谱分离

以甲醇-磷酸溶液梯度洗脱

流速1 mL.min-1

检测波长254 nm

柱温30℃

进样体积10μL。结果:没食子酸和槲皮素分别在15.0～180（r=0.999 6）

1.25～15.0 mg.L-1（r=0.999 7）线性关系良好;平均回收率分别为98.2%和99.2%

RSD分别为0.7%和0.5%。结论:方法操作简便

精密度高

结果准确

可同时测定猴耳环胶囊中没食子酸与槲皮素的含量

可用于猴耳环胶囊的质量控制。

Abstract

Objective:To establish a HPLC method for determination of gallic acid and quercetin in Houerhuan Xiaoyan Jiaonang.Method: The analytical column was Hypersil BDS-C18（4.6 mm×150 mm

5 μm）

and the mobile phase was methanol-phosphate solution with the gradient elution.The flow rate was 1.0 mL · min-1 and the detection wavelength was 254 nm.The column temperature was kept at 30 ℃

and the sample volumn was 10 μL.Result: A good linearity was obtained in the range of 15.0-180.0 mg · L-1（r=0.999 6） and 1.25-15.0 mg · L-1（r=0.999 7） for gallic acid and quercetin respectively

and the average recovery was 98.2%（RSD 0.7%） and 99.2%（RSD 0.5%） respectively.Conclusion: The method is convenient

accurate

precise and can be used in the content determination of gallic acid and quercetin in Pithecellobium clypearia antiphlogistic capsule at the same time

and can be used for the quality control of this capsule.

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CHEN Yan-wei

ZHANG Huan

YANG Shi-lei

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LIANG Hui

PENG Liang

LI Yi-guang

CHEN Jie

Related Institution

Guangxi Institute of Chinese Medicine and Pharmaceutical Sciences

Guangxi Key Laboratory of Traditional Chinese Medicine Quality Standards

School of Pharmaceutical Sciences,Guangxi Medical University

Drugs R&D Centre,Guangxi Traditional Chinese Medical University

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