Objective:To revise and improve quality standard of Solanum nigrum.Method:According to the research methods of pharmaceutical standard in 2010 version of ’Chinese Pharmacopoeia’ Appendix

S.nigrum was identified by TLC and microscopy.Moisture

ash and acid insoluble ash from 10 batches of S.nigrum were detected

and the content of active ingredients were determined.Result:Microscopic characteristics of S.nigrum was derermined

identification methods of TLC was established;The content of moisture could not more than 10%

acid insoluble ash could not more than 3.0%;Solasonine was linear in the range of 1.002-20.04 μg

but solamargine was linear in 1.023-20.46 μg.Conclusion:The total content of solasonine and solamargine from S.nigrum in different areas had significant difference.This established method could accurately reflect the inherent quality of S.Nigrum

which could be used as the amendments of quality standard for S.nigrum.