Simultaneous Determination of Rutin,Forsythoside A and Phillyrin of Forsythiae Fructus and its Extract by HPLC

WEI Yue; LI Xiao; LI Zi-hong; FAN Yi; ZHANG Hai-yan; ZHAO Tian-zeng

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Simultaneous Determination of Rutin,Forsythoside A and Phillyrin of Forsythiae Fructus and its Extract by HPLC

更新时间：2024-09-23

- Simultaneous Determination of Rutin,Forsythoside A and Phillyrin of Forsythiae Fructus and its Extract by HPLC
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 19, Issue 9, Pages: 108-111(2013)
- 作者机构：
  
  河南省生物技术开发中心
- 作者简介：
- 基金信息：
- DOI：
  CLC： R284.1
- Published：2013
- 稿件说明：
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[1]魏悦,李晓,李自红,范毅,张海艳,赵天增.HPLC同时测定连翘药材及提取物中芦丁、连翘酯苷A和连翘苷的含量[J].中国实验方剂学杂志,2013,19(09):108-111.

WEI Yue, LI Xiao, LI Zi-hong, et al. Simultaneous Determination of Rutin,Forsythoside A and Phillyrin of Forsythiae Fructus and its Extract by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(9): 108-111.
[1]魏悦,李晓,李自红,范毅,张海艳,赵天增.HPLC同时测定连翘药材及提取物中芦丁、连翘酯苷A和连翘苷的含量[J].中国实验方剂学杂志,2013,19(09):108-111. DOI：

WEI Yue, LI Xiao, LI Zi-hong, et al. Simultaneous Determination of Rutin,Forsythoside A and Phillyrin of Forsythiae Fructus and its Extract by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(9): 108-111. DOI：

摘要

目的:建立连翘药材及提取物中芦丁、连翘酯苷A和连翘苷的含量测定方法。方法:应用高效液相色谱-二极管阵列检测法（HPLC-DAD）

根据各类成分紫外吸收光谱的差异

分别在354

329

277 nm波长下检测芦丁、连翘酯苷A和连翘苷。采用Sunfire C18（4.6 mm×150 mm

5μm）色谱柱

流动相为乙腈（A）-0.2%冰醋酸溶液（B）

梯度洗脱（0 min

14%A;15min

18%A;40 min

30%A）

流速1 mL.min-1

柱温40℃。结果:芦丁、连翘酯苷A和连翘苷分别在0.098～4.9

0.24～12.0

0.1～5.0μg线性关系良好

平均回收率分别为98.63%

98.97%

102.18%

RSD分别为7.94%

0.93%

1.63%。结论:方法精密度高、重复性好

能够应用于连翘药材及提取物的质量控制。

Abstract

Objective:To establish a HPLC method for simultaneous determination of rutin

forsythoside A and phillyrin of Forsythiae Fructus and its extract.Method: The procedure of HPLC-PDA was performed on the chromatographic column of Sunfire C18（4.6 mm×150 mm

5 μm）

and the mobile phase in the gradient elution（0 min

14%A;15 min

18%A;40 min

30%A） was acetonitrile（A）-0.2% acetic acid solution（B）.The flow rate was 1.0 mL · min-1;the temperature was kept at 40 ℃

and the detection wavelength was set at 354

329 nm and 277 nm respectively.Result: Rutin

forsythoside A and phillyrin were linear in the range of 0.1-2.0

0.12-2.4 μg and 0.05-1.0 μg respectively.The average recoveries were 98.63%

98.97% and 102.18% respectively（RSD 7.94%

0.93% and 1.63%）.Conclusion: The method is accurate with higher repeatability

which can be used in the quality control of Forsythiae Fructus and its extract.

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Related Author

WEI Yue

LI Xiao

LI Zi-hong

FAN Yi

ZHANG Hai-yan

ZHAO Tian-zeng

ZHANG Fei

TIAN Su

Related Institution

Beijing Research Institute of Chinese Medicine， Beijing University of Chinese Medicine

College of Life Sciences， Beijing University of Chinese Medicine

College of Chinese Materia Medica， Beijing University of Chinese Medicine

College of Pharmacy, Shaanxi University of Chinese Medicine

Guizhou Provincial Engineering Center of Efficient Utilization of Natural Medicinal Resources, High Educational Key Laboratory of Natural Medicinal Pharmacology and Druggability of Guizhou Province, Joint Key Laboratory of Guizhou Medical University-Guiyang City, Key Laboratory of Optimal Utilization of Natural Medicinal Resources, School of Pharmaceutical Sciences, Guizhou Medical University

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