Objective: To establish HPLC fingerprint of Tibetan medicine Pterocephali Herba

and compare it of Pterocephali Herba from different place

provided a new methods for its quality evaluation. Method: Adoption the method of HPLC to determine 10 groups of Pterocephali Herba from different place. Chromatographic Column was Boston Symmetrix(250×4.6mm

5μm)

mobile phase was Methanol-0.1 mol.L-1 ammonium acetate solution(81:19)

velocity was 0.8mL.min-1

the detection wavelength was 210nm

column temperature was 30℃. And compared the similarity of Pterocephali Herba from different place. Result: Taking advantage of < USP fingerprint similarity evaluation system (2004A version)> to establish common mode of HPLC fingerprint of Tibetan medicine Pterocephali Herba

and marked 101 characteristic peaks. The 10 groups samples had high similarity. Conclusion: This method had good repeatability

precision

and stability

providing scientific basis for quality control of Pterocephali Herba.