Comparative Study on the Characteristic Chromatogram of Different Processing Methods of Ilex hainanensis Merr.

XIE Zhen; ZHANG Long; CHEN Yong; ZHENG Xi-ren; LI Yao-hua; NONG Li; LIU Ding; LI Yi-xuan

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Comparative Study on the Characteristic Chromatogram of Different Processing Methods of Ilex hainanensis Merr.

更新时间：2024-01-04

- Comparative Study on the Characteristic Chromatogram of Different Processing Methods of Ilex hainanensis Merr.
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 20, Issue 23, (2014)
- 作者机构：
  
  广西中医药大学药学院,柳州市人民医院,广西中医药大学药学院,广西中医药大学药学院,广西中医药大学药学院,广西中医药大学药学院,广西中医药大学药学院,广西中医药大学药学院
- 作者简介：
- 基金信息：
- DOI：
  CLC：
- Published：2014
- 稿件说明：
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XIE Zhen, ZHANG Long, CHEN Yong, et al. Comparative Study on the Characteristic Chromatogram of Different Processing Methods of Ilex hainanensis Merr.[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(23).
DOI：

XIE Zhen, ZHANG Long, CHEN Yong, et al. Comparative Study on the Characteristic Chromatogram of Different Processing Methods of Ilex hainanensis Merr.[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(23). DOI：

摘要

[目的]采用HPLC法研究不同炮制工艺山绿茶药材特征图谱的变化

为科学评价和控制其质量提供有效的方法。[方法]以70%乙醇为提取溶剂

采用加热回流的方法提取制备供试样品。以Phenomenon C18色谱柱为固定相

乙腈-0.1%磷酸水溶液为流动相进行梯度洗脱

检测波长330nm

柱温25℃

进样量10μl

流速1.0ml/min

进行HPLC特征图谱检测。以芦丁为对照品

检测不同炮制工艺药材芦丁含量变化。[结果]不同炮制工艺药材中共确定9个主要共有特征峰。研究发现特征图谱中主要色谱峰发生了显著改变

与炮制前相比峰面积显著降低。表明山绿茶药材经炮制处理后

主要化学成分含量明显减少。[结论]建立了一种重现性、精密度良好的HPLC特征图谱检测方法

可用于山绿茶药材及其炮制品的质量控制。

Abstract

Objective To establish a sensitive and specific HPLC method for quality control of Ilex hainanensis Merr.．Methods HPLC method was applied for quality assessment of Ilex hainanensis Merr.． HPLC analysis was performed on a HanbonC18 column (250mm×4．6mm，5 m)．The mobile phase consisted of acetonitrile (solvent B) and watercontaining0.1% (v/v) phosphoric acid (solvent A) at a constant flow rate of 1.0ml/min．An increasing linear gradient (v/v) of solvent B was used. The injection volume was 10μl. The column temperature was set at 25℃. The chromatogram s were monitored at 330nm. Results In different varieties of processing methods of Ilex hainanensis Merr. showing 9 characteristic peaks. It was established from its prepared samples of Ilex hainanensis Merr.．Conclusion The characteristic chromatogram of Ilex hainanensis Merr. with high specificity can be used to control its quality as well as the consistency of processing products.

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Related Author

XIE Zhen

ZHANG Long

CHEN Yong

ZHENG Xi-ren

LI Yao-hua

NONG Li

LIU Ding

LI Yi-xuan

Related Institution

Beijing Research Institute of Chinese Medicine， Beijing University of Chinese Medicine

College of Life Sciences， Beijing University of Chinese Medicine

College of Chinese Materia Medica， Beijing University of Chinese Medicine

College of Pharmacy, Shaanxi University of Chinese Medicine

Guizhou Institute for Food and Drug Control

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