UHPLC Fingerprint of Lonicera japonica Thunb. from Different Areas

HAN Yong-Cheng; LIU Wei; CHEN Ning; CUI Yong-Xia; HUANG Li-Jie

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UHPLC Fingerprint of Lonicera japonica Thunb. from Different Areas

更新时间：2024-01-04

- UHPLC Fingerprint of Lonicera japonica Thunb. from Different Areas
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 20, Issue 2, (2014)
- 作者机构：
  
  河南中医学院分析测试中心,河南中医学院分析测试中心,河南中医学院分析测试中心,河南中医学院分析测试中心,河南中医学院分析测试中心
- 作者简介：
- 基金信息：
- DOI：
  CLC：
- Published：2014
- 稿件说明：
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HAN Yong-Cheng, LIU Wei, CHEN Ning, et al. UHPLC Fingerprint of Lonicera japonica Thunb. from Different Areas[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(2).
DOI：

HAN Yong-Cheng, LIU Wei, CHEN Ning, et al. UHPLC Fingerprint of Lonicera japonica Thunb. from Different Areas[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(2). DOI：

摘要

目的建立不同产地金银花药材的超高效液相特征性指纹图谱

为有效控制和科学评价金银花药材整体质量提供依据。方法采用AgilentC18 色谱柱(50 mm?2.1 mm

1.8μm)

流动相为乙腈-0.2%磷酸水

以0.4mL/min的流速进行梯度洗脱

检测波长238 nm

柱温30℃

。结果在21min内得到金银花药材的指纹图谱

对其中5个色谱峰进行了初步归属

并对14批药材样品进行了分析

其相似度为0.915~0.987。结论UHPLC指纹图谱方法较HPLC大大缩短了分析时间

可用于金银花药材的质量评价。

Abstract

Objective To develop an UHPLC method of determining the characteristic chromatographic profile of Lonicera japonica Thunb for controlling the drug quality quickly and accurately. Methods The analysis was performed on an Agilent C18 column(50 mm?2.1 mm

1.8μm) with acetonitrile－0.2%phosphoric acid as mobile phase in gradient elution mode. The flow rate was 0.4 ml.min-1and the injection volume was 2.0μl with the detection wavelength of238 nm．The column temperature was set at 30℃. Results The fingerprint of Lonicera japonica Thunb was obtained within 21min and five peaks in the fingerprint were designed.14 samples were analyzedand the similarities were 0.915-0.987. Conclusion the UHPLC system enjoys the merits of a higher speed

sensitivity

and resolution

It can be used to control the quality of Lonicera japonica Thunb.

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Related Institution

Institute of Chinese Materia Medica，China Academy of Chinese Medical Sciences

State Key Laboratory of Southwestern Traditional Chinese Medicine（TCM） Resources， School of Pharmacy，Chengdu University of TCM

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Tianjin University of Traditional Chinese Medicine

Modern Research Center for Traditional Chinese Medicine，Shanxi University

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