SHANG Lei, OUYANG Zhen, ZHAO Ming, et al. HPLC-MS/MS Analysis and Determination of Alkaloids of Morus alba L. Leaves[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(2).
DOI:
SHANG Lei, OUYANG Zhen, ZHAO Ming, et al. HPLC-MS/MS Analysis and Determination of Alkaloids of Morus alba L. Leaves[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(2).DOI:
HPLC-MS/MS Analysis and Determination of Alkaloids of Morus alba L. Leaves
Objective: To use liquid chromatography-mass spectrometry techniques for qualitative analysis of alkaloids of Morus alba L. leaves and determine the content of total alkaloids in alkaloids in Morus alba L. leaves by HPLC-UV with pre-column derivatization. Method: The chromatographic separation was performed on a HiQSiL C18 column(250 mm×4.6 mm
5 μm) using acetonitrile and 0.1% acetic acid aqueous solution under isocratic elution(50:50
V/V) at a flow rate of 0.8 mL.min-1. Morus alba L. leaves alkaloids was analyzed with 9-fluorenylmethyl chlorformate(FMOC-Cl) as the pre-column derivative agent. Detection wavelength was set at 254 nm with electrospray ionization source(ESI) and positive ion mode. And 1-deoxynojirimycin as the external standard to calculate mulberry leaf total alkaloid content. Result: Six alkaloid compounds were identified as DNJ
fagomine
3-epi-fagomine
DAB
Calystegine B2 and 2-O-β-Glc-DAB.The content of 3 batches test samples were more than 75% by external standard method. Conclusion: The method is sensitive
accurate
simple
and reliable. It can be used for quantitative analysis of Morus alba L. leaves alkaloids and preparation.
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Related Author
SHANG Lei
OUYANG Zhen
ZHAO Ming
WANG Ji-biao
WANG Fan
WANG Yuan
DUAN Haohan
JIA Hao
Related Institution
Henan Engineering Research Center for Quality Control and Evaluation of Traditional Chinese Medicine(TCM),School of Pharmacy,Henan University of Chinese Medicine
Luohe Medical College
Third Affiliated Hospital of Henan University of Chinese Medicine
Tianjin University of Traditional Chinese Medicine
Institute of Chinese Materia Medica,China Academy of Chinese Medical Sciences