Study on Quality Standard of Hedyotis Diffusae Wild. in the Qingshen Granule

SHI Jin-min; WEI Liang-bin; LI Rao-rao; JIA Lu; ZHANG Zhi-jie; GU Xue-zhu

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Study on Quality Standard of Hedyotis Diffusae Wild. in the Qingshen Granule

更新时间：2024-01-04

- Study on Quality Standard of Hedyotis Diffusae Wild. in the Qingshen Granule
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 20, Issue 1, (2014)
- 作者机构：
  
  1. 中国中医学科学院中药研究所
  2. 郑州大学药学院
  3. 安徽中医学院第一附属医院,安徽中医学院第一附属医院,中国中医学科学院中药研究所,郑州大学药学院,中国中医学科学院中药研究所,中国中医学科学院中药研究所
- 作者简介：
- 基金信息：
- DOI：
  CLC：
- Published：2014
- 稿件说明：
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SHI Jin-min, WEI Liang-bin, LI Rao-rao, et al. Study on Quality Standard of Hedyotis Diffusae Wild. in the Qingshen Granule[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(1).
DOI：

SHI Jin-min, WEI Liang-bin, LI Rao-rao, et al. Study on Quality Standard of Hedyotis Diffusae Wild. in the Qingshen Granule[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(1). DOI：

摘要

[摘要] 目的: 建立清肾颗粒中白花蛇舌草的质量控制标准。方法: 采用薄层色谱法对制剂中的白花蛇舌草进行定性鉴别，采用高效液相色谱法测定制剂中白花蛇舌草的去乙酰车叶草酸甲酯的含量。色谱条件：色谱柱为Kromasil C18 （4.6 mm ? 250 mm，5 μm），流动相为乙腈-水（3.5: 96.5），检测波长236 nm，流速1.0 mL?min-1。结果：薄层色谱清晰，阴性无干扰，重现性良好，去乙酰车叶草酸甲酯在0.042 ~ 1.35 4 μg呈良好的线性关系（r = 1.000 0）平均回收率99.96%（RSD 1.66%，n = 6）。结论：该方法可操作性强，重复性好，可用于清肾颗粒中白花蛇舌草的质量控制。

Abstract

[Abstract] Objective: To study on quality standard of Hedyotis Diffusae Wild. in the Qingshen granule. Method:Thin-layer chrmatography was used to identidy Hedyotis Diffusae Wild.

The content of Deacetyl asperulosidicacid methyl ester was determined by HPLC. The HPLC consisted of Kromasil C18 (4.6 mm ? 250 mm

5 μm)

acetonitrile-water (3.5: 96.5) as mobile phase

flow rate of 1.0 mL?min-1

detection wavelength at 276 nm. Result:The study on the quality control showed that the characteristic of identification by HPLC was distinct

accurate and reproducible.The linear range of Deacetyl asperulosidicacid methyl ester was 0.042 μg ~ 1.35 4 μg(r = 1.000 0). the average recovery was 99.96% With RSD of 1.66%(n = 6). Conclusion: The methods are accurate and quick in qualitative identification and quantitative assay of this preparation

can be used for the quality control of Qingshen granule.

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Related Author

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JIA Lu

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XU Fuyu

LUO Hongbing

ZHONG Lingyun

Related Institution

Jiangxi University of Chinese Medicine

Jiangxi Institute of Quality and Standardization

School of Pharmaceutical Sciences， Changchun University of Chinese Medicine

The 3rd Affiliated Hospital of Changchun University of Chinese Medicine

Institute of Chinese Materia Medica，China Academy of Chinese Medical Sciences

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