Objective: To investigate the chemical constituents of the ethyl acetate fraction of ethanol extract of Euscaphis japonica. Method: Various preparative chromatographic techniques were used for the isolation and purification of the compounds and their structures were determined by comparison of their chromatographic and spectral data with those of the authentic samples and those reported in the literature. Result: Seven compounds were obtained and identified as vanillin(1)

vanillic acid(2)

5-hydroxymethylfurfural(3)

sinapic aldehyde(4)

oleanolic acid(5)

gallic acid(6)

and protocatechuic acid(7). Conclusion: Compounds 2-7 were isolated from the genus Euscaphis for the first time.