Objective:To invastigate the site of analgesic effect of the total saponin from Thladiantha dubia root (TSRT). Method: Kunming mice 120 were devided into formalin test and improved Randall-Selitto method grougs

60 in each groug. Sixty mices were divided into TSRT 240

120

60 mg·kg-1grougs

Asp 200 mg·kg-1groug

Mor 5 mg·kg-1

grpop and NS group. The Mor group was given by sc

the others were given by ig.The site of analgesic effect of TSRT respectively was analysed by formalin test and mend Randall-Selitto method in mice. Result: In formalin test in mice

TSRT of 120

60 mg·kg-1 significantly lowered the pain score of the second phase(P＜0.05 vs NS group)

but there is no effect on the pain score of the first phase(P＞0.05 vs NS group)

TSRT of 240 mg·kg-1 lowered both the pain scores of the first phase and second phase(P＜0.05-0.01 vs NS group). In imoroved Randall-Selitto method in mice

TSRT markedly increased the pain threshold value on inflammation foot(P＜0.01 vs NS group)

but pain threshold value in normal foot did not obviously elevated(P＞0.05 vs NS group). Conclusion: The site of analgesic effect of TSRT is mainly located in the periphery.