Optimization of Purification Technology for Effective Parts from Compound Tongmai Granules by AB-8 Macroporous Absorption Resin
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Optimization of Purification Technology for Effective Parts from Compound Tongmai Granules by AB-8 Macroporous Absorption Resin
Chinese Journal of Experimental Traditional Medical FormulaeVol. 19, Issue 5, Pages: 4-6(2013)
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Published:2013
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CHEN Chao, CHEN Guo-qin, CHEN A-li, et al. Optimization of Purification Technology for Effective Parts from Compound Tongmai Granules by AB-8 Macroporous Absorption Resin[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(5): 4-6.
DOI:
CHEN Chao, CHEN Guo-qin, CHEN A-li, et al. Optimization of Purification Technology for Effective Parts from Compound Tongmai Granules by AB-8 Macroporous Absorption Resin[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(5): 4-6.DOI:
Optimization of Purification Technology for Effective Parts from Compound Tongmai Granules by AB-8 Macroporous Absorption Resin
Objective:To optimize purification technology of compound Tongmai granules by macroporous resin. Method: Taking adsorption-desorption rate of total phenolic acid and puerarin as indexes
effect of the concentration of sample solution
diameter-height ratio
elution agent on purification technology of compound Tongmai granules was investigated by single factor test. Result: AB-8 macroporous absorption resin was the best resin
optimum purification technology was as following:the concentration of sample solution 110 g·L-1
diameter-height ratio of resin column 1∶10
ratio of resin-material drug 2.8∶1(adsorbed 3 h)
eluted with 2 BV water
then eluted with 6 BV 50% ethanol
collected eluent. Conclusion: This optimized purification technology was reasonable and stable
it could be extended to large-scale production applications.
Optimization of Purification Technology for Effective Parts from Compound Tongmai Granules by AB-8 Macroporous Absorption Resin
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Related Author
CHEN Chao1
CHEN Guo-qin2
CHEN A-li1
CUI Hong-hua1
GUO Jia-wen1
WANG Shu-mei1
YANG Xin
SONG Jian-ping
Related Institution
Henan University of Traditional Chinese Medicine
School of Chinese Materia Medica, Guangdong Pharmaceutical University
Institute of Medicinal Plant Development,Chinese Academy of Medical Sciences and Peking Union Medical College
Institute of Chinese Materia Medica,China Academy of Chinese Medical Sciences
School of Pharmacy, Jiangxi University of Chinese Medicine