Determination of Psoralen of Radix Fici in Different Leave Type by HPLC

WANG Xiao-ping; HUANG Xiang; LU Qi-feng; CEN Ye-wen

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Determination of Psoralen of Radix Fici in Different Leave Type by HPLC

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- Determination of Psoralen of Radix Fici in Different Leave Type by HPLC
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 19, Issue 5, Pages: 93-95(2013)
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- Published：2013
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WANG Xiao-ping, HUANG Xiang, LU Qi-feng, et al. Determination of Psoralen of Radix Fici in Different Leave Type by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(5): 93-95.
DOI：

WANG Xiao-ping, HUANG Xiang, LU Qi-feng, et al. Determination of Psoralen of Radix Fici in Different Leave Type by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(5): 93-95. DOI：

摘要

目的: 建立不同叶型五指毛桃中补骨脂素含量的高效液相色谱测定方法。方法: 采用Extend-C18色谱柱(4.6 mm×250 mm

5 μm);流动相乙腈-水 35∶65;流速1.0 mL·min-1;柱温 35 ℃

检测波长245 nm。结果: 补骨脂素对照品在1.04~104 mg·L-1范围内呈良好曲线关系

线性回归方程 Y=72.150 6X+133.483 0

r=0.999 1

平均加样回收率为93.6%

RSD 1.975%。结论: 不同叶型的五指毛桃中补骨脂素的含量有差异

该方法操作简便、准确性高、重复性好

可用于五指毛桃药材的质量评价。

Abstract

Objective:To establish the method by HPLC for determination of psoralen in different leave type of Radix Fici. Method: HPLC was conducted with a Extend-C18(4.6 mm×250 mm

5 μm) column

the mobile phase was acetonitrile-water(35∶65)with the flow rate of 1.0 mL·min-1. The column temperature was 35 ℃ and the detection wavelength was set at 245 nm. Result: Psoralen's linearity was good in a range of 1.04-104 mg·L-1

The linear regression standard curve was Y=72.150 6X+133.483 0(r=0.999 1) and the average recovery was 96.3% with RSD 1.975%. Conclusion: Psoralen is different in quantity of different leave type of Radix Fici. This method is simply and accurate

and has good repeatability. It could be used to quality evaluation method for Radix Fici.

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Related Author

WANG Xiao-ping1

HUANG Xiang1

LU Qi-feng2

CEN Ye-wen1

FAN Xiao-wen

MA Qing-he

LI Hai-qi

DAI Xiu-yong

Related Institution

Yulin Normal University

College of Agronomy and Biotechnology,Southwest University

广西中医药大学赛恩斯新医药学院,广西中医药大学,广西中医药大学赛恩斯新医药学院

成都中医药大学,九江学院,重庆市中药研究院重庆市中药资源重点实验室,重庆市中药研究院重庆市中药资源重点实验室,重庆市中药研究院重庆市中药资源重点实验室

Dongcheng District Institute for Drug Control

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