Optimization of Purification Technology for Total Flavonoids from
|更新时间:2024-01-04
|
Optimization of Purification Technology for Total Flavonoids from
Chinese Journal of Experimental Traditional Medical FormulaeVol. 19, Issue 6, Pages: 11-14(2013)
作者机构:
作者简介:
基金信息:
DOI:
CLC:
Published:2013
稿件说明:
移动端阅览
ZHONG Kun-rui, ZHANG Fan, JIANG Yan-yan, et al. Optimization of Purification Technology for Total Flavonoids from [J]. Chinese journal of experimental traditional medical formulae, 2013, 19(6): 11-14.
DOI:
ZHONG Kun-rui, ZHANG Fan, JIANG Yan-yan, et al. Optimization of Purification Technology for Total Flavonoids from [J]. Chinese journal of experimental traditional medical formulae, 2013, 19(6): 11-14.DOI:
Optimization of Purification Technology for Total Flavonoids from
Objective: To optimize polyamide purification technology of total flavonoids from Mentha haplocalyx. Method: With the content and transfer rate of total flavonoids as indexes
adsorption capacity of 5 different kinds of purification materials for total flavonoids was investigated by single factor test
in order to determine optimum purification material
and adsorption
impurity removing and eluting conditions in purification technology were optimized. Result: Optimum purification conditions were as follows:The concentration of sample solution 0.1 g·mL-1
diameter-height ratio of column 1:9
adsorption rate 2 BV·h-1
proportion between the total amount of solution and resin column 1:6
3 BV of 10% ethanol to wipe off impurity with flow rate of 4 BV·h-1
then 3 BV of 90% ethanol to elute with 8 BV·h-1.After purified by polyamide(30-60 mesh)
purity of total flavonoids was up to 50%. Conclusion: This optimized purification technology was simple and feasible with good reproducibility
it could be used for the separation and the enrichment of total flavonoids from M.haplocalyx.
Purification of Total Flavones from by Macroporous Resins
Purification Process of Total Flavonoids from with Macroporous Resins
Purification Technology of Total Flavonoids from Yushen Granules by Macroporous Resin
Purification of Total Flavonoids from Leaves of Macroporous Resin
Optimization of Purification Process for Total Flavonoids from by Macroporous Adsorption Resin
Related Author
XIAO Ting
YAN Zhi-yong
ZUO Chang-ying
PAN Ling-zhen
ZHANG Zhan-ping
ZENG Yong-chang
LIANG Shao-yu
XING Xue-feng
Related Institution
Key Unit of Exploring Effective Substances of Classical and Famous Prescription,State Administration of Traditional Chinese Medicine of the People’s Republic of China
Beijing University of Chinese Medicine
Southen University Key Lab of Traditional Chinese Medicine and New Drug,Guangdong Provincial Key Lab of Traditional Chinese Medicine
Pharmacy Technology Center,Tsinghua University
Center of Pharmacy R&D,XinJiang Huachun Bio-Pharmaceutical Co. Ltd.