Study on Fingerprint of Smilacis Glabrae Rhizoma Formula Granules by HPLC

WEN Qing-wei; ZOU Lu-hui; LAN Xiao-qing; HUANG Li-feng; LU Dong; WEI Hong-yan; ZHOU Yong-qiang

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Study on Fingerprint of Smilacis Glabrae Rhizoma Formula Granules by HPLC

更新时间：2024-01-04

- Study on Fingerprint of Smilacis Glabrae Rhizoma Formula Granules by HPLC
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 19, Issue 6, Pages: 99-102(2013)
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- Published：2013
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WEN Qing-wei, ZOU Lu-hui, LAN Xiao-qing, et al. Study on Fingerprint of Smilacis Glabrae Rhizoma Formula Granules by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(6): 99-102.
DOI：

WEN Qing-wei, ZOU Lu-hui, LAN Xiao-qing, et al. Study on Fingerprint of Smilacis Glabrae Rhizoma Formula Granules by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(6): 99-102. DOI：

摘要

目的: 采用反相高效液相色谱法(HPLC)

建立土茯苓配方颗粒的指纹图谱。方法: 采用Ultimate® AQ-C18(4.6 mm×250 mm

5 μm)色谱柱

甲醇-0.2%磷酸水溶液(35:65)洗脱;检测波长290 nm;流速1.0 mL·min-1

进样量10 μL

采集时间60 min。结果: 分析方法有较好的重复性

共检出7个共有峰

并指认出落新妇苷(astilbin)、白藜芦醇(resveratrol)2个化学成分。10批土茯苓配方颗粒的指纹图谱相似度均>0.98。结论: HPLC指纹图谱重复性好

可作为土茯苓配方颗粒产品质量控制的技术手段之一。

Abstract

Objective: To investigate and establish the fingerprint of Smilacis Glabrae Rhizoma Formula Granules by RP-HPLC. Method: The samples were injected with 10 μL in a HPLC system coupled with Ultimate® AQ-C18 column(4.6 mm×250 mm

5 μm)

and eluted with methanol-0.2% phosphoric acid solution(35: 65) at 1.0 mL·min-1 under 30 ℃.The data was recorded for 60min by UV director at 290 nm. Result: Two compounds(astilbin

resveratrol)were identified from the 7 common peaks in the fingerprint which had a good reproducibility and similarity with correlation coefficient (>0.98) in the analysis of 10 different batches products. Conclusion: HPLC fingerprint would be a selective

simple

acute and suitable method for quality control in the herbs and Formula Granules of Smilacis Glabrae Rhizoma.

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Related Institution

Institute of Chinese Materia Medica，China Academy of Chinese Medical Sciences

State Key Laboratory of Southwestern Traditional Chinese Medicine（TCM） Resources， School of Pharmacy，Chengdu University of TCM

Sichuan Neo-green Pharmaceutical Technology Development Co. Ltd.，Pengzhou

Shaanxi University of International Trade&Commerce

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