Atmospheric Pressure Chemistry Ionization Mass Spectrum Analysis of Gingkolides in Extract
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Atmospheric Pressure Chemistry Ionization Mass Spectrum Analysis of Gingkolides in Extract
Chinese Journal of Experimental Traditional Medical FormulaeVol. 19, Issue 5, Pages: 155-158(2013)
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Published:2013
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GONG Li-li, KONG De-gang, SUN Guo-ming, et al. Atmospheric Pressure Chemistry Ionization Mass Spectrum Analysis of Gingkolides in Extract[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(5): 155-158.
DOI:
GONG Li-li, KONG De-gang, SUN Guo-ming, et al. Atmospheric Pressure Chemistry Ionization Mass Spectrum Analysis of Gingkolides in Extract[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(5): 155-158.DOI:
Atmospheric Pressure Chemistry Ionization Mass Spectrum Analysis of Gingkolides in Extract
摘要
目的: 用高效液相色谱-大气压化学电离-质谱(HPLC-APCI-MS)法对银杏叶提取物中银杏内酯进行分析。 方法: 液相色谱条件Agilent 3000 Extend-C18色谱柱(4.6 mm×250 mm
5μm)
流动相 甲醇-水 30∶70
流速 0.8 mL·min-1
检测波长 220 nm
柱温 35 ℃;负离子模式检测。 结果: 解析鉴别了白果内酯和银杏内酯A
B
C
J
其分子离子峰的m/z分别为324.8
407.1
422.9
438.9
422.9。 结论: 为银杏内酯的检测提供了一种快速有效的质谱方法。
Abstract
Objective:To study gingkolides in Ginkgo biloba extract by HPLC-APCI-MS. Method: Analysis of gingkolides was done by HPLC-APCI-MS in negative ion mode. The separation was performed on Agilent 3000 Extend-C18 column with methanol-water (30∶70) as mobile phase
and the flow rate was 0.8 mL·min-1. The spectrometer was APCI-MS. Result: Bibobalide
ginkgolide A
B
C
J were identified
the m/z of molecular ion peak is respectively 324.8
407.1
422.9
438.9
422.9. Conclusion :The established method by HPLC-APCI-MS can be adopted for sensitive and rapid identification of gingkolides.