Dynamic Changes of SP mRNAs in Lung Tissues of Rabbits Models with Differentiated Syndromes of Weifen, Qifen, Yingfen, and Xuefen

DENG Shi-gui; YE Yin-yi; PENG Zhen-xiang

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Dynamic Changes of SP mRNAs in Lung Tissues of Rabbits Models with Differentiated Syndromes of Weifen, Qifen, Yingfen, and Xuefen

更新时间：2024-01-04

- Dynamic Changes of SP mRNAs in Lung Tissues of Rabbits Models with Differentiated Syndromes of Weifen, Qifen, Yingfen, and Xuefen
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 19, Issue 6, Pages: 279-282(2013)
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- Published：2013
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DENG Shi-gui, YE Yin-yi, PENG Zhen-xiang. Dynamic Changes of SP mRNAs in Lung Tissues of Rabbits Models with Differentiated Syndromes of Weifen, Qifen, Yingfen, and Xuefen[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(6): 279-282.
DOI：

DENG Shi-gui, YE Yin-yi, PENG Zhen-xiang. Dynamic Changes of SP mRNAs in Lung Tissues of Rabbits Models with Differentiated Syndromes of Weifen, Qifen, Yingfen, and Xuefen[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(6): 279-282. DOI：

摘要

目的: 探讨卫气营血辨证肺损伤时肺表面活性蛋白SP-A

SP-B

SP-C mRNA表达随时间变化的规律。方法: 30只新西兰兔随机分为正常对照组和卫气营血辨证肺损伤组(2 h代表卫分组

6 h代表气分组

12 h代表营分组

24 h代表血分组

各1组)

每组6只采用耳缘静脉推注脂多糖(LPS)建立卫气营血辨证肺损伤模型

注射LPS后处死各组动物

采用RT-PCR法检测肺组织SP-A

SP-B

SP-C mRNA表达变化。正常对照组麻醉后取肺组织标本待测。结果: 与正常对照组相比

卫气营血辨证肺损伤卫分、气分、营分、血分各亚组SP-A

SP-B

SP-C mRNA表达显著下降(P<0.05)。SP-C气分组与卫分组相比mRNA表达无显著性差异

其余卫气营血辨证肺损伤各亚组间SP-A

SP-B

SP-C mRNA表达均具有显著性差异(P<0.05)

并随注射LPS时间延长

SP mRNA表达明显降低

其表达与肺损伤程度呈负相关。结论: 卫气营血辨证肺损伤兔肺表面活性蛋白SP-A

SP-B

SP-C mRNA表达呈时间依赖性降低

是卫气营血辨证肺损伤的重要物质基础之一。

Abstract

Objective: To study the dynamic changes of SP mRNAs in lung tissue of animal models with differentiated syndromes of Weifen

Qifen

Yingfen

and Xuefen. Method: Thirty rabbits were randomly divided into normal control group and Weifen

Qifen

Yingfen

and Xuefen groups(2

24 h four subgroups

corresponding to Weifen

Qifen

Yingfen and Xuefen

n=6 each)

Lipopolysaccharides(LPS

1 mg·kg-1)was given by intravenous injection through auricular vein in the groups.Rabbits in each subgroup were killed after injection

lung tissue specimens were collected and tested.Expressions of SP-A

SP-B

SP-C mRNA at different points of time were measured by using real-time PCR.The control group was received no treatment

lung tissue specimen was tested as in the treatment group. Result: Compared with the control group

levels of transcription of SP-A

SP-B

SP-C mRNA were decreased significantly in Weifen

Qifen

Yingfen and Xuefen groups(P<0.05).Levels of transcription of SP-C mRNA showed no significant changes in Weifen and Qifen groups

besides

levels of transcription of SP-A

SP-B

SP-C mRNA among every two subgroups in Weifen

Qifen

Yingfen and Xuefen groups were decreased significantly(P<0.05).In addition

the expression of SP mRNAs was decreased significantly as time passed after the injection of LPS

and the expression was negatively correlated with the degree of lung injury. Conclusion: The changes of the SP-A

SP-B

SP-C mRNA in rabbits following acute lung injury are decreased in a time-dependent manner

which maybe one of the important material basis of acute lung injury induced by syndromes of Weifen

Qifen

Yingfen

and Xuefen.

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