Effect of Dihuang Yinzi on Expression of Bax,Bcl-2 and Caspase-3 Proteins in Rat Model of Cerebral Ischemia Reperfusion

GONG Jian-wei; YE Lei; FAN Qiao-ling

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Effect of Dihuang Yinzi on Expression of Bax,Bcl-2 and Caspase-3 Proteins in Rat Model of Cerebral Ischemia Reperfusion

更新时间：2024-09-23

- Effect of Dihuang Yinzi on Expression of Bax,Bcl-2 and Caspase-3 Proteins in Rat Model of Cerebral Ischemia Reperfusion
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 19, Issue 5, Pages: 248-251(2013)
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- Published：2013
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GONG Jian-wei, YE Lei, FAN Qiao-ling. Effect of Dihuang Yinzi on Expression of Bax,Bcl-2 and Caspase-3 Proteins in Rat Model of Cerebral Ischemia Reperfusion[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(5): 248-251.
DOI：

GONG Jian-wei, YE Lei, FAN Qiao-ling. Effect of Dihuang Yinzi on Expression of Bax,Bcl-2 and Caspase-3 Proteins in Rat Model of Cerebral Ischemia Reperfusion[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(5): 248-251. DOI：

摘要

目的: 探讨地黄饮子对脑缺血再灌注模型大鼠Bax蛋白、Bcl-2 蛋白、Caspase-3 蛋白表达的影响及其保护大脑损伤的机制。方法: SD大鼠60只

随机分为6组

即假手术组、模型组、阳性药尼莫地平组(1 mg·kg-1)和地黄饮子高、中、低(32

8 g·kg-1)剂量组

每组10只

用药7 d后造模。用结扎大鼠双侧颈总动脉方法建立脑缺血再灌注模型

观察地黄饮子对大鼠脑缺血再灌注120 min后脑组织Bax 蛋白、Bcl-2 蛋白、Caspase-3 蛋白的表达的影响。结果: 模型组大鼠Bax 蛋白表达升高、Bcl-2 蛋白表达降低、Caspase-3 蛋白表达升高

与假手术组有显著差异(P<0.05)

各药物治疗组能显著降低Bax 蛋白表达、升高Bcl-2 蛋白表达、降低Caspase-3 蛋白表达

与模型组有显著差异(P<0.05) 。结论: 地黄饮子能通过下调Bax 蛋白、上调Bcl-2 蛋白和下调Caspase-3 蛋白表达

抑制脑缺血再灌注损伤后细胞凋亡

从而对脑缺血再灌注损伤起到保护作用。

Abstract

Objective:To explore the effect of Dihuang Yinzi on the expression of Bax

Bcl-2 and Caspase-3 proteins and protective cerebral mechanism after cerebral ischemia reperfusion. Method: Sixty SD rats were selected and randomized into six groups: sham group

model group and high

medium

low dose groups of Dihuang Yinzi(32

8 g·kg-1) and nimodipine positive drug group(1 mg·kg-1)

each involved ten rats. The cerebral ischemia/reperfusion model was established. The expression of Bax

Bcl-2 and Caspase-3 proteins were detected 120 min after cerebral ischemia/reperfusion treated by Dihuang Yinzi. Result: The expression of Bax and Caspase-3 proteins in the models were significantly higher than those in the sham group (P<0.05). The expression of Bcl-2 proteins in the model group were significantly lower than those in the sham group (P<0.05). While the expression of Bax and Caspase-3 proteins in the treatment group were significantly lower than those in the model group (P<0.05)

the expression of Bcl-2 proteins in the treatment group were significantly higher than those in the model group (P<0.05). Conclusion: Dihuang Yinzi can upregulate the expression of Bcl-2 protein and downregulate the expression of Bax and Caspase-3 proteins. Dihuang Yinzi may protect the cerebral injury induced by ischemia/reperfusion through inhibiting neuron apoptosis.

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Related Author

GONG Jian-wei1

YE Lei2

FAN Qiao-ling1

ZHANG Xiao-li

CHENG Xiang

LIU Yang

SHI Xue-kui

GU Hao

Related Institution

Nanjing University of Chinese Medicine

Binzhou Medical University

Tianjin University of Traditional Chinese Medicine

Henan University of Chinese Medicine

Department of Pharmacy,Jiangsu University

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