Objective:To establish the method for determining nvzhenoside in Liuwei Wuling tablet by HPLC. Method: Nvzhenoside was analyzed by HPLC. The optimal conditions of separation and detection were achieved on a Agilent ZORBAX SB (4.6 mm×250 mm

5 μm) by isocratic elution with the mobile phase of methanol-water (30∶70) at the flow rate of 1.0 mL·min-1. The detective wavelength of deoxyschizandrin was set at 224 nm. Result: The regression equation of nvzhenoside was Y=1 233 200X+2.476 4×104(r=0.999 9). So the linearity was acceptable. The average recovery of nvzhenoside was 99.96% (RSD 0.58%). Conclusion: This method was simple

rapid and feasible for the quantification of nvzhenoside in Liuwei Wuling tablet by HPLC.