Determination of Conduriol A in the Flower of

QIU Qin; ZHEN Han-shen; HUANG Pei-qian; LI Wen-xiu

您当前的位置：

首页 >

文章列表页 >

Determination of Conduriol A in the Flower of

更新时间：2024-01-04

- Determination of Conduriol A in the Flower of
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 19, Issue 6, Pages: 155-158(2013)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：
  CLC：
- Published：2013
- 稿件说明：
移动端阅览
QIU Qin, ZHEN Han-shen, HUANG Pei-qian, et al. Determination of Conduriol A in the Flower of [J]. Chinese journal of experimental traditional medical formulae, 2013, 19(6): 155-158.
DOI：

QIU Qin, ZHEN Han-shen, HUANG Pei-qian, et al. Determination of Conduriol A in the Flower of [J]. Chinese journal of experimental traditional medical formulae, 2013, 19(6): 155-158. DOI：

摘要

目的: 对匙羹藤花中的有效成分牛弥菜醇A的含量测定进行研究

为科学评价其质量提供科学依据。方法: 采用正交实验优选匙羹藤花样品溶液的制备方法

同时采用高效液相色谱法(HPLC)测定牛弥菜醇A的含量

以氨基柱(4.6 mm×250 mm

5 μm)为色谱柱

乙腈-水(90:10)为流动相

流速1.0 mL·min-1

检测波长207 nm

柱温:30 ℃。结果: 匙羹藤花样品溶液的最佳制备方法为150倍50%甲醇超声提取50 min

牛弥菜醇A在0.400 4～20.02 μg与峰面积成良好的线性关系(r=0.999 7)

平均回收率为98.98%

RSD 1.15%(n=6)。结论: 采用HPLC建立的方法准确、快速、灵敏度高、重复性好

可作为匙羹藤花的质量控制方法。

Abstract

Objective: To study the determination of conduritol A in the flower of Gymnema sylvestre. Method: The best extraction technology for conduritol A was studied through orthogonal experiment; HPLC was adopted to determine the content of conduritol A.The separation was performed on a Thermo NH2 column(4.6 mm×250 mm

5 μm); acetonitrile:water(90: 10)was used as mobile phase; the flow rate was 1.0 mL·min-1; the detection wave was set at 207 nm and the column temperature was kept at 30 ℃. Result: The best extraction technology of conduritol A was ultrasonic extraction in 50% methanol of 150 times for 50 min.The liner range of conduritol A was 0.400 4-20.02 μg(r=0.999 7); the average recovery rate was 98.98%

RSD was 1.15%(n=6). Conclusion: The method was accurate

quick

sensitive with good reproducibility

it could be used as the quality control method of the flower of Gymnema sylvestre.

关键词

Keywords

references

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

etermination of Geniposide and Liquiritigenin in Legan-Qingyan Capsules by HPLC

Determination of Harpagoside in Granules of Yangyin Qinfei by RP-HPLC

Determination of Corilagin and Gallic Acid in Yiganjiedu Oral Liquid by HPLC

Determination of 5-hydroxymethylfurfural in Xiaobanxia Tang

Related Author

ZHANG Ke-wei

WU Hao

CUI Xiao-bing

WANG Mei

SONG Shen

CHEN Hua-guo

ZHOU Xin

ZHAO Chao

Related Institution

Chengde Jingfukang Pharmaceutical Group Co.Ltd,New Excipients of Traditional Chinese MedicineEngineering Research Center of Hebei Province

Jiangxi College of Traditional Chinese Medicine

Chengdou University of Tradition Chinese Medicine,Chengdou

Guangxi Traditional Chinese Medical University

Dali University,Dali

AI问答

Postal code：100700
Tel：010-84076882 Email：syfjx_2010@188.com
Technical support is provided by Beijing Founder electronics co., LTD 京ICP备11006657号-10 京公网安备11010802024621
It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.

⁰