Study of Mailuoning Cause an Allergic the RBL-2H3 Cells

YU Hai-bin; LIU Qing; CUI Lin

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Study of Mailuoning Cause an Allergic the RBL-2H3 Cells

更新时间：2024-09-23

- Study of Mailuoning Cause an Allergic the RBL-2H3 Cells
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 19, Issue 3, Pages: 245-248(2013)
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- Published：2013
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YU Hai-bin, LIU Qing, CUI Lin. Study of Mailuoning Cause an Allergic the RBL-2H3 Cells[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(3): 245-248.
DOI：

YU Hai-bin, LIU Qing, CUI Lin. Study of Mailuoning Cause an Allergic the RBL-2H3 Cells[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(3): 245-248. DOI：

摘要

目的: 通过研究临床含有绿原酸的中药注射剂脉络宁针剂对RBL-2H3细胞(大鼠嗜碱性白血病细胞系)脱颗粒、释放介质的影响

探讨绿原酸与中药注射剂发生严重过敏反应的关系

为临床安全用药提供依据。方法: 以含有绿原酸的中药针剂脉络宁为研究对象

以不含绿原酸的葛根素针剂为阴性对照

以绿原酸对照品为阳性对照

通过对体外培养RBL-2H3细胞模型的作用

观察该细胞模型发生脱颗粒反应和细胞形态变化

检测β-己糖胺酶释放量。结果: 脉络宁、绿原酸组可显著引起RBL-2H3细胞释放β-己糖胺酶(P<0.05);葛根素注射液组能不同程度促进β-己糖胺酶的释放。结论: 脉络宁组可显著引起RBL-2H3细胞释放β-己糖胺酶

提示绿原酸与中药注射剂脉络宁发生严重过敏反应有密切关系。

Abstract

Objective:Relativity between the severe allergic reaction of the chlorogenic acid and traditional chinese medicine injection was studied by observing influnce of Mailuoning injection on release media of RBL-2H3 cell (rat basophilic leukemia cell line). Method: Mailuoning containing chlorogenic acid was as a suject

and puerarin injection not containing as a negative control

while chlorogenic acid standard as a positive control

the influntion of RBL-2H3 cells model in vitro

the degranulation response and changes in cell morphology was observed

β-hexosaminidase release was detected. Result: The Mailuoning and chlorogenic acid can be a significant factor resulting in release of β-hexosaminidase from RBL-2H3 cells (n=5

P<0.05)

puerarin injection group could promote the release of β-hexosaminidase. Conclusion: The Mailuoning group abversely caused the RBL-2H3 cells releasing β-hexosaminidase

and there is some relativity between the severe allergic reaction of the chlorogenic acid and traditional chinese medicine injection.

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Related Author

LIU Qing2

CUI Lin1

YU Hai-bin1

TANG Jia-ming

ZHAO Yuan

LIU Jiong

LI Yong-mei

DENG Xiang-liang

Related Institution

The First Affiliated Hospital of Pharmacology Three Laboratories,Henan College of Traditional Chinese Medicine

Henan Graduate School of Traditional Chinese Medicine

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