Study of TG in CTGF Abnormal Expression and the Effect in the Renal Cortex of DM Rats
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Study of TG in CTGF Abnormal Expression and the Effect in the Renal Cortex of DM Rats
Chinese Journal of Experimental Traditional Medical FormulaeVol. 18, Issue 24, Pages: 307-311(2012)
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Published:2012
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CHANG Jing-zhi, WANG Chen, SHEN Yong-jie, et al. Study of TG in CTGF Abnormal Expression and the Effect in the Renal Cortex of DM Rats[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(24): 307-311.
DOI:
CHANG Jing-zhi, WANG Chen, SHEN Yong-jie, et al. Study of TG in CTGF Abnormal Expression and the Effect in the Renal Cortex of DM Rats[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(24): 307-311.DOI:
Study of TG in CTGF Abnormal Expression and the Effect in the Renal Cortex of DM Rats
Objective:To study the mechanism of tripterygium glycosides(TG) in protecting the kidney by observing the effection of TG in abnormal expression of connective tissue growth factor(CTGF) and some factors of kidney function in artificial diabetes rats. Method: Forty-five Male SD rats were randomly divided into normal control group
diabetic nephropathy(DN) and diabetic nephropathy group treated with tripterygium glycosides(TG) group
1.8 g·kg-1·d-1 ig
once a day
using 8 weeks) DN group and TG group rats were intraperitoneal injected with sacrificed. The rats were sacrificed after 4 and 8 weeks. The body weight
blood glucose
blood urea nitrogen(BUN)
creatinine(Cr) and 24 h urinary protein were measured in each rat. The pathology changing of the rat kidney was observed by HE staining. The expression of CTGF protein in kidney was measured by immunohistochemistry. Result: Compared with control group
the level of blood glucose
BUN
Cr and 24 h urinary protein were significantly increased in DN group (P<0.01).The expression of CTGF protein was increased in DN group compared with control group(P<0.01); After intervention with tripterygium glycosides
these indexes were all significantly declined except blood glucose (P<0.05
P<0.01). Conclusion: The TG on reducing the urinary protein in DM rats and improving renal injury in diabetic nephropathy may related to the inhibition of renal CTGF expression.
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