Objective:To study the protective effect of total flavonoids from leaves of Lonicera Japonica (TFLLJ) on acute hepatic injury induced by carbon tetrachloride (CCl4 ) in mice. Method: Sixty mice were randomly divided into six groups: the normal group

the model group

high-dose TFLLJ group (400 mg·kg-1)

middle-dose TFLLJ group (200 mg·kg-1) and low-dose TFLLJ group (100 mg·kg-1)

bifendate group (150 mg·kg-1). Except for normal group and model group

the other mice were orally administered corresponding drugs once per day for ten days. Then

the treated groups and model group were ip given 0.1% CCl4 (0.01 mL·g-1) once to produce acute liver injury on the tenth day. The liver injury was evaluated by the indexes of serum alanine aminotransferase (AST)

aspartate aminotransferase (ALT)

liver index (LI)

liver superoxidedismutase (SOD) and malondialdehyde (MDA)

and the morphological changes of liver tissue. Result: FLLJ(400

200

100 mg·kg-1)could remarkably resist the increase of ALT and AST

decrease the MDA level and could improve SOD activity (P<0.01 or P<0.05 versus model group). Furthermore

TFLLJ could ameliorate the hepatic pathological changes. Conclusion: TFLLJ have protective effects on acute hepatic injury induced by CCl4 in mice. It may be related to its anti-oxidative effect for lipids.