Optimization of Purification Technology of Total Flavonoid from by D101 Macroporous Resin
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Optimization of Purification Technology of Total Flavonoid from by D101 Macroporous Resin
Chinese Journal of Experimental Traditional Medical FormulaeVol. 19, Issue 2, Pages: 38-40(2013)
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Published:2013
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LIAO Chun-yan, YANG Xin-lv. Optimization of Purification Technology of Total Flavonoid from by D101 Macroporous Resin[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(2): 38-40.
DOI:
LIAO Chun-yan, YANG Xin-lv. Optimization of Purification Technology of Total Flavonoid from by D101 Macroporous Resin[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(2): 38-40.DOI:
Optimization of Purification Technology of Total Flavonoid from by D101 Macroporous Resin
Objective: To optimize purification technology conditions of total flavonoid from Abrus cantoniensis by D101 macroporous resin. Method: With rutin as reference
the content of total flavonoid was determined by UV colorimetry
with adsorption rate and desorption rate as indexes
conditions of absorption and desorption were investigated by single factor test. Result: The best technology conditions were as follows:the concentration of sample solution 3 g· L-1
sample flow rate 3 BV·h-1
with 2 BV 80% ethanol as eluant
pH 6.0
elution flow rate 3.5 BV·h-1.Under these conditions
purity of total flavonoid was increased from 17.95% to 52.80%. Conclusion: D101 macroprous resin had good purification effect for total flavonoid from A. cantoniensis