Influence of Qigong Kangxian Decoction and its Component Herbs on HSC-T6 Cell Growth and TGF- mRNA Expression
|更新时间:2024-09-23
|
Influence of Qigong Kangxian Decoction and its Component Herbs on HSC-T6 Cell Growth and TGF- mRNA Expression
Chinese Journal of Experimental Traditional Medical FormulaeVol. 19, Issue 3, Pages: 249-251(2013)
作者机构:
作者简介:
基金信息:
DOI:
CLC:
Published:2013
稿件说明:
移动端阅览
CHEN Gui-min, LIANG Zhen-yu, GAO Yuan. Influence of Qigong Kangxian Decoction and its Component Herbs on HSC-T6 Cell Growth and TGF- mRNA Expression[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(3): 249-251.
DOI:
CHEN Gui-min, LIANG Zhen-yu, GAO Yuan. Influence of Qigong Kangxian Decoction and its Component Herbs on HSC-T6 Cell Growth and TGF- mRNA Expression[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(3): 249-251.DOI:
Influence of Qigong Kangxian Decoction and its Component Herbs on HSC-T6 Cell Growth and TGF- mRNA Expression
Objective:To analyze the mechanism of antifibrotic role of Qigong Kangxian decoction (QKD) and the component herbs in rat models of hepatic fibrosis induced by immune injury. Method: A modified comparison method of serum pharmacology was applied to analyze
and QKD's impact on growth of hepatic stellate cell(HSC-T6) cell and expression of transforming growth factor (TGF-β1) receptor mRNA protein. Result: significant increase in cell growth of stellate cell(HSC-T6) were observed in the rat models comparing with that of normal healthy rats. While rat models treated with different QKD doses showed significant inhibitory effects on growth of HSC-T6 cell (P<0.01 or P<0.05) comparing with model rats. Comparing with normal control group
TGF-β1 mRNA concentration increased significantly in rat models (P<0.01)
but in rat models treated with QKD
TGF-β1 mRNA concentration were all regulated to decrease obviously (P<0.01). Conclusion: One of the main mechanisms of antifibrotic role of QKD and individual herbs is the inhibitory impact on HSC cell growth
and down-regulation of TGF-β1mRNA receptor protein expression. QKD shows a dose dependent effect
which is better than that of Huoxue formula and Ruanjian formula.