Objective: To observe the influence of total flavones of Epimedium leptorrhizum Stearn on hFOB1.19 osteoblast activity. Method: The hFOB1.19 osteoblast was treated with 50

25

12.5

6.25

3.13

1.56

0.78

0.39

0.20 mg· L-1 total flavones of E. leptorrhizum Stearn

and then detected proliferation rate by MTT and alkaline phosphate(ALP) secretion by automatic biochemical analyzer after 72 h(single administration) and 1

4

7

10 d(continuous administration). Result: 0.78-50 mg· L-1 total flavones of E. leptorrhizum Stearn can promote proliferation of hFOB1.19 osteoblast by single administration after 72 h. The max proliferation rate is 132.32%. 0.78 mg· L-1 total flavones can also promote the ALP secretion obviously. Total flavones of E. leptorrhizum Stearn has no influence on hFOB1.19 osteoblast by continuous administration at 1-4 d

but shows a different degree of promoting effect after 7 d. From 0.78 to 25.0 mg· L-1

the effect presents an obvious dose relationship. With the further increase of drug concentration

the cell proliferation no longer increasing

and the effect lasted at least until 10 days. 12.5

25.0

50.0 mg· L-1 total flavones of E. leptorrhizum Stearn at the 7 d and 25.0

50.0 mg· L-1 total flavones at the 10 d can promote ALP secretion significantly. Conclusion: The total flavones of E. leptorrhizum Stearn can promote hFOB1.19 osteoblast proliferation and ALP secretion. It shows that the promotion of osteoblast activity by total flavones of E. leptorrhizum Stearn maybe a pathway of prevention and treatment of osteoporosis.