Objective: According to the study on dynamic changes of three flavonoid C-glycosides constituents in different harvest time of trollius chninesis

the best harvest time for trollius chninesis was established

providing a scientific basis to achieve the greatest medicinal value. Method: Using a HPLC method for gradient elution

the mobile phase was a mixture of acetonitrile-0.35% phosphoric acid(14:86)

and the detection wavelength was set at 345 nm. The content of orientin

vetixin

2"-O-β-L-galactopyranosyl orientin in different harvest time was tested. Result: Three flavonoid C-glycosides in trollius chinesis in different periods reached to the top after 3 to 6 days when blossomed. Conclusion: 3 to 6 days after blossom of trollius chinesis is considered to be the best harvest time. This method is simple with high precision and good repeatability

which offered a scientific basis to achieve the greatest medicinal value.