Analysis Several Active Ingredients of Zhitong Prescription One-time by RP-HPLC Determination

SHI Jan-nan; YANG Yi-ying; LIU Yan-jie

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Analysis Several Active Ingredients of Zhitong Prescription One-time by RP-HPLC Determination

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- Analysis Several Active Ingredients of Zhitong Prescription One-time by RP-HPLC Determination
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 19, Issue 1, Pages: 136-138(2013)
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- Published：2013
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SHI Jan-nan, YANG Yi-ying, LIU Yan-jie. Analysis Several Active Ingredients of Zhitong Prescription One-time by RP-HPLC Determination[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(1): 136-138.
DOI：

SHI Jan-nan, YANG Yi-ying, LIU Yan-jie. Analysis Several Active Ingredients of Zhitong Prescription One-time by RP-HPLC Determination[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(1): 136-138. DOI：

摘要

目的: 利用多波长反相高效液相色谱法(RP-HPLC)建立对民间验方仲氏止痛散加减后的止痛方中6种有效成分的含量进行同时测定的方法。方法: 使用Diamond C18色谱柱(4.6 mm×250 mm

5 μm)

柱温30℃

甲醇-乙腈-0.1%氨水为流动相

以1 mL·min-1梯度洗脱

检测波长230 nm(芍药苷)、235 nm(乌头碱、次乌头碱)、280 nm(延胡索乙素)、280 nm(原阿片碱)、235 nm(新乌头碱)。结果: 6个成分峰面积与浓度呈良好的线性(r>0.999 4)

芍药苷、延胡索乙素、原阿片碱、乌头碱、次乌头碱、新乌头碱的加样回收率分别为97.2%(RSD 1.4%)

96.5%(RSD 1.2%)

102.5%(RSD 0.7%)

96.8%(RSD 0.7%)

100.8%(RSD 1.1%)

98.5%(RSD 1.1%)。结论: 利用RP-HPLC同时测定止痛方中的几种有效成分

简便易行

为止痛方相关制剂的质量控制提供一定的依据。

Abstract

Objective: Using reversed-phase high performance liquid chromatography (RP-HPLC) method to analyze 6 ingredients of Zhitong prescription one-time. Method: Using Diamond C18 column (4.6 mm×250 mm

5 μm)

the column temperature was 30℃; methanol acetonitrile 0.1% ammonia water as the mobile phase

1 mL·min-1 gradient elution; detection wavelength was 230 nm (paeoniflorin)

235 nm (the aconitine hypaconitine)

280 nm (tetrahydropalmatine)

280 nm (protopine) and 235 nm (mesaconitine). Result: The peak area and concentration of the six components showed good linearity (r>0.999 4) the paeoniflorin aconitine hypaconitine

tetrahydropalmatine protopine

new aconitine the recoveries were 97.2% (RSD 1.4%)

96.5% (RSD 1.2%)

102.5% (RSD 0.7%)

96.8%(RSD 0.7%)

100.8%(RSD 1.1%)

98.5%(RSD 1.1%). Conclusion: By using RP-HPLC simultaneous determination of the active ingredients of the the analgesic prescription of several simple

we can provide a basis for the quality control of the preparation of Zhitong prescription.

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