Optimization of Purification Technology for Total Flavonoids in   by Macroporous Adsorption Resin

SANG Lin; WANG Xiao-lin; ZHONG Fang-li; ZHANG Zhi-long; XUE Jian-fei; CHEN Shuai

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Optimization of Purification Technology for Total Flavonoids in by Macroporous Adsorption Resin

更新时间：2024-01-04

- Optimization of Purification Technology for Total Flavonoids in by Macroporous Adsorption Resin
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 19, Issue 6, Pages: 57-60(2013)
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- Published：2013
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SANG Lin, WANG Xiao-lin, ZHONG Fang-li, et al. Optimization of Purification Technology for Total Flavonoids in by Macroporous Adsorption Resin[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(6): 57-60.
DOI：

SANG Lin, WANG Xiao-lin, ZHONG Fang-li, et al. Optimization of Purification Technology for Total Flavonoids in by Macroporous Adsorption Resin[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(6): 57-60. DOI：

摘要

目的: 优选独活总黄酮的大孔吸附树脂纯化工艺。方法: 以总黄酮的吸附率和洗脱率为指标

采用静态吸附-洗脱与动态吸附-洗脱试验优选大孔树脂型号;通过单因素试验优选独活总黄酮的纯化工艺参数。结果: LSA-33型大孔吸附树脂纯化效果最好

其最佳工艺为上样药液中总黄酮质量浓度0.1 g·mL-1

上柱药液pH 2～3

吸附速率4 BV·h-1

用3 BV 75%乙醇洗脱

洗脱速率3 BV·h-1

收集洗脱液

经大孔树脂纯化后独活干浸膏中总黄酮纯度由原来的9.87%提高至32.59%。结论: LSA-33型大孔树脂适用于独活总黄酮的初步纯化。

Abstract

Objective: To optimize purification technology of total flavonoids in Angelica pubescens by macroporous resin. Method: With adsorption rate and desorption rate of total flavonoids as indexes

macroporous resin models were optimized by static adsorption and desorption experiments as well as dynamic adsorption and desorption tests;Purification technology parameters of total flavonoids were optimized by single factor test. Result: LSA-33 macroporous resin presented the best effect of purification

optimum purification conditions were as followings:the concentration of total flavonoids from sample solution 0.1 g·mL-1

pH of sample solution 2-3

adsorption rate 4 BV·h-1

eluted with 3 BV 75% ethanol

desorption rate 3 BV·h-1

collected eluent

purity of total flavonoids in dry extract of A.pubescens was changed from 9.87% to 32.59%. Conclusion: LSA-33 macroporous resin was suitable for preliminary purification of total flavonoids in A.pubescens.

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Related Institution

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