Objective:To establish an HPLC-DAD method for simultaneous determination of rutin

quercetin and quercitroside in Incarvillea mairei. Method: A Dikma Diamonsil C18column (4.6 mm×150 mm

5 μm) was used. The column temperature was set at 30 ℃; the investigated compounds were separated with gradient mobile phase consisting of methanol (A)-0.5% phosphoric acid (B); the flow rate was 1.0 mL·min-1 and the detection wavelength was kept at 254 nm. Result: The good separation of three flavonoids was achieved within 10 min. The linearity was achieved in the range of 0.30-1.0 mg (r=0.999 8) for rutin

0.030-0.10 mg (r=0.999 8) for quercetin and 0.010-0.033 mg (r=0.999 5) for quercitrosid. The average recoveries were 100.95% (RSD 0.98%)

99.51% (RSD 1.54%) and 102.84% (RSD 3.53%) respectively. Conclusion: The method is simple

rapid and accurate

which can be able to detect rutin

quercetin and quercitroside simultaneously

and can be used for quality control of I. mairei.