Optimization of Separation and Purification Technology for Total Flavonoids and Puerarin from by Macroporous Adsorption Resin
|更新时间:2024-01-04
|
Optimization of Separation and Purification Technology for Total Flavonoids and Puerarin from by Macroporous Adsorption Resin
Chinese Journal of Experimental Traditional Medical FormulaeVol. 19, Issue 4, Pages: 23-26(2013)
作者机构:
作者简介:
基金信息:
DOI:
CLC:
Published:2013
稿件说明:
移动端阅览
ZHU Meng-liang, ZHAO Li-jun, LIANG Xin-li, et al. Optimization of Separation and Purification Technology for Total Flavonoids and Puerarin from by Macroporous Adsorption Resin[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(4): 23-26.
DOI:
ZHU Meng-liang, ZHAO Li-jun, LIANG Xin-li, et al. Optimization of Separation and Purification Technology for Total Flavonoids and Puerarin from by Macroporous Adsorption Resin[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(4): 23-26.DOI:
Optimization of Separation and Purification Technology for Total Flavonoids and Puerarin from by Macroporous Adsorption Resin
Objective: To optimize separation and purification technology of total flavonoids and puerarin from Pueraria lobata by macroporous adsorption resin. Method: Static adsorption and desorption performance of four kinds of different polar macroporous adsorption resins(S-8
AB-8
D-101
HPD-100) for total flavonoids and puerarin from P. lobata were investigated
in order to select optimal resin;With yield and purity of total flavonoids and puerarin as indexes
single factor test was used to investigate adsorption and elution conditions. Result: AB-8 macroporous resin was chosen
its optimized technology conditions were as followings:the concentration of sample solution 1.0 g·mL-1
mass ratio of raw material-resin 1:2
diameter-height ratio of column 1:6
washed by 2 BV water
eluted with 2 BV 80% ethanol
elution flow rate 2 mL·min-1.Elution rate of total flavonoids and puerarin were more than 92%
purity of total flavonoids and puerarin could reach 80% and 25%
respectively. Conclusion: AB-8 macroporous adsorption resin was sutiable for enrichment total flavonoids and puerarin from P. lobata
it was a kind of ideal separation and purification medium
Separation and Purification Technology of Pseudo-ginsenoside F from Total Saponins in Leaves and Stems of
Effect of Salvianolic Acid B Combined with Puerarin on Pyroptosis of SH-SY5Y Cells Injured by Oxygen-glucose Deprivation/Reoxygenation
Active Ingredients,Product Development and Breeding of Medicinal Cannabis: A Review
Structures Characteristics and Bioactivity of Polysaccharide CALB-2 from Aurantii Fructus
Investigation of Adsorption Properties of Five Macroporous Resins for Total Flavonoids in Epimedii Folium
Related Author
TIAN Xu
REN Yuan-yuan
LI Ping-ya
WANG Cui-zhu
LIU Jin-ping
TU Shuxin
WANG Yan
HE Xiaoli
Related Institution
Institute of Medicinal Plant Development,Chinese Academy of Medical Sciences and Peking Union Medical College
Institute of Chinese Materia Medica,China Academy of Chinese Medical Sciences
College of Food Science and Biology, Hebei University of Science and Technology
Guangdong Standardized Processing Engineering Technology Research Center of Traditional Chinese Medicine(TCM),School of TCM, Guangdong Pharmaceutical University
School of Pharmaceutical Sciences, Guangzhou University of Chinese Medicine