Optimization of Preparation Technology for Phosphorylated Polysaccharides from by Response Surface Methodology
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Optimization of Preparation Technology for Phosphorylated Polysaccharides from by Response Surface Methodology
Chinese Journal of Experimental Traditional Medical FormulaeVol. 19, Issue 6, Pages: 50-53(2013)
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Published:2013
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NING Yuan-ling, MENG Fa-yan, JIANG Jie, et al. Optimization of Preparation Technology for Phosphorylated Polysaccharides from by Response Surface Methodology[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(6): 50-53.
DOI:
NING Yuan-ling, MENG Fa-yan, JIANG Jie, et al. Optimization of Preparation Technology for Phosphorylated Polysaccharides from by Response Surface Methodology[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(6): 50-53.DOI:
Optimization of Preparation Technology for Phosphorylated Polysaccharides from by Response Surface Methodology
Objective: To optimize preparation technology of phosphorylated polysaccharides from Dimocarpus longan. Method: Phosphorylation reaction was used to prepare phosphorylated polysaccharides from D.longan by using POCl3
with degree of substitution as index
which was determined by phosphomolybdate blue method
a Box-Behnken central composite design of response surface methodology design and single factor test were used to investigate three factors influencing preparation technology
such as volume ratio of pyridine-POCl3
reaction temperature and time. Result: Optimum preparation technology was as following:volume ratio of pyridine-POCl3 3:1
reaction temperature 0 ℃
reaction time 60 minutes
a higher degree of substitution of 0.018 4 was obtained. Conclusion: This optimized preparation technology was stable and feasible
it could be extended to application in industrial production.
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