Determination of Catechin in by RP-HPLC

JIA Zhi-ruo; LI Bing; LI Yao-hua; ZHEN Han-shen; LU Cheng-sheng

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Determination of Catechin in by RP-HPLC

更新时间：2024-09-23

- Determination of Catechin in by RP-HPLC
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 19, Issue 5, Pages: 117-119(2013)
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- Published：2013
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JIA Zhi-ruo, LI Bing, LI Yao-hua, et al. Determination of Catechin in by RP-HPLC[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(5): 117-119.
DOI：

JIA Zhi-ruo, LI Bing, LI Yao-hua, et al. Determination of Catechin in by RP-HPLC[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(5): 117-119. DOI：

摘要

目的: 建立测定杜仲中儿茶素含量的高效液相色谱方法。方法: 设计正交试验对样品的前处理过程进行优化

采用反相高效液相色谱法测定杜仲中儿茶素含量

色谱柱为Agilent HC-C18 (4.6 mm×250 mm

5 μm);流动相乙腈-0.4%磷酸溶液(13∶87);流速1.0 mL·min-1

检测波长280 nm。结果: 儿茶素在9.620~194.0 mg·L-1线性关系良好(r=0.999 3)

平均加样回收率为97.38%

RSD 2.1%。结论: 该法简便快速、准确性和重复性好

可用于杜仲中儿茶素的含量测定。

Abstract

Objective:To establish a HPLC method for the determination of catechin in Eucommia ulmoides. Method: The orthogonal test was used to optimize the pretreatment process. RP-HPLC was adopted

on a column of Agilent HC-C18(4.6 mm×250 mm

5 μm)

using the mobile phase of acetonitrile-0.4% phosphoric acid solution (13∶87)

at a flow rate of 1.0 mL·min-1; the detection wavelength was set at 280 nm. Result: Catechin was linear in the range of 9.620-194.0 μg·mL-1(r=0.999 3). The average recovery of catechin was 97.38% with RSD 2.1%. Conclusion: The method is simple

rapid and reliable with good reproduciblity

which can be used for the content determination of catechin in E. ulmoides.

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Related Author

JIA Zhi-ruo1

LI Bing1

LI Yao-hua1

ZHEN Han-shen1

LU Cheng-sheng1

GENG Dan

MA Wen-Fang

ZHEN Han-shen

Related Institution

Guangxi University of Chinese Medicine

Chengdu University of Traditional Chinese Medicine

School of Pharmaceutical Sciences， Changchun University of Chinese Medicine

Jilin Ginseng Academy，Changchun University of Chinese Medicine

Sunflower Pharmaceutical Group（Xiangyang） Longzhong Co. Ltd.，Xiangyang

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