Objective:To develop a HPLC method for determination of four indicative components (paconiflorin

naringin

hesperidin and neohesperidin) in decoction of Zhishao powders. Method: Syncronis C18 (4.6 mm×250 mm

5 μm) was adopted; and the mobile phase was 0.3% phosphoric acid solution (A)-acetonitrile (B) with gradient elution at a flow rate of 1.0 mL·min-1

and the detection wavelength was 230 nm (0-30 min) for paconiflorin

283 nm (30-60 min) for naringin

hesperidin

neohesperidin. Result: The content of five indicative components in decoction of Zhishao powders was stable. The method had a good linearity in the ranges of 0.12-1.73 μg (r=0.999 6) for paconiflorin

0.38-5.68 μg (r=0.999 9) for naringin

0.64×10-1-0.96 μg (r=0.999 9) for hesperidin

0.72-10.83 μg (r=0.999 7) for neohesperidin. The average recoveries was 104.17%

100.98%

103.72% and 101.01% respectively. Conclusion: This method is dependable

simple and practical

and may be use to control the quality of Zhishao powders.